中国医学创新
中國醫學創新
중국의학창신
MEDICAL INNOVATION OF CHINA
2015年
5期
110-112
,共3页
徐小娟%刘英志%陈碧莲%林静俐
徐小娟%劉英誌%陳碧蓮%林靜俐
서소연%류영지%진벽련%림정리
氟化钠%五倍子水提取物%变形链球菌%防龋
氟化鈉%五倍子水提取物%變形鏈毬菌%防齲
불화납%오배자수제취물%변형련구균%방우
Sodium fluoride%Gallnut water extract%Streptococcus mutans%Anti-caries
目的：研究氟化物与五倍子水提取物联合应用对变形链球菌生长和代谢的影响。方法：取10 mL的无菌培养管120支，分为12组，对照组1组，实验组11组，每组各10支。对照组中含无菌去离子水，实验组中含有不同浓度氟化钠（0.01%、0.02%）、五倍子水提取物（0.25、0.5、1 mg/mL）及氟化钠与五倍子联合应用的培养基内培养变形链球菌标准菌株，检测培养前后两组培养基光密度值及pH的变化。结果：与对照组相比，各实验组变链菌的生长受到明显抑制，同时各组培养基的pH变化也逐渐减小，氟化钠与五倍子联合应用时未发现明显的协同效应。结论：氟化钠及五倍子水提取物均可显著抑制变形链球菌的生长及产酸代谢活动，两者联合应用时未见明显的协同效应。
目的：研究氟化物與五倍子水提取物聯閤應用對變形鏈毬菌生長和代謝的影響。方法：取10 mL的無菌培養管120支，分為12組，對照組1組，實驗組11組，每組各10支。對照組中含無菌去離子水，實驗組中含有不同濃度氟化鈉（0.01%、0.02%）、五倍子水提取物（0.25、0.5、1 mg/mL）及氟化鈉與五倍子聯閤應用的培養基內培養變形鏈毬菌標準菌株，檢測培養前後兩組培養基光密度值及pH的變化。結果：與對照組相比，各實驗組變鏈菌的生長受到明顯抑製，同時各組培養基的pH變化也逐漸減小，氟化鈉與五倍子聯閤應用時未髮現明顯的協同效應。結論：氟化鈉及五倍子水提取物均可顯著抑製變形鏈毬菌的生長及產痠代謝活動，兩者聯閤應用時未見明顯的協同效應。
목적：연구불화물여오배자수제취물연합응용대변형련구균생장화대사적영향。방법：취10 mL적무균배양관120지，분위12조，대조조1조，실험조11조，매조각10지。대조조중함무균거리자수，실험조중함유불동농도불화납（0.01%、0.02%）、오배자수제취물（0.25、0.5、1 mg/mL）급불화납여오배자연합응용적배양기내배양변형련구균표준균주，검측배양전후량조배양기광밀도치급pH적변화。결과：여대조조상비，각실험조변련균적생장수도명현억제，동시각조배양기적pH변화야축점감소，불화납여오배자연합응용시미발현명현적협동효응。결론：불화납급오배자수제취물균가현저억제변형련구균적생장급산산대사활동，량자연합응용시미견명현적협동효응。
Objective:To study the effects of combined application of fluoride and the gallnut water extract on the growth and acidogenic metabolism of Streptococcus mutans(S.mutans).Method:10 mL of sterile culture tube 120 were selected and divided into 12 groups,1 group was control group,and 11 groups were experimental group,each group of 10. The experimental group standard S. mutans were cultured in vitro in the TS medium which contained 5%sucrose and different concentration of NaF(0.01%,0.02%)and/or gallnut water extract(0.25,0.5,1 mg/mL),and the medium without neither NaF nor gallnut water extract act as control group. The optical density(OD)value of the medium and pH change before and after the culture was determined. Result:Compared with the control group,the experimental group S.mutans growth was obviously inhibited,and with the increase of amount of sodium fluoride and gallnut water extract concentration of culture,the number of S.mutans showed a concentration dependent decrease;with the increase of sodium fluoride and the culture medium of gallnut water extract concentration,pH changes of medium of gradually reduced,NaF combined with gallnut water extract showed no obvious synergistic effect. Conclusion:NaF and gallnut water extract could significantly inhibit the growth and acidogenic metabolism of S. mutans. No obvious synergistic effect was observed while the NaF combined with gallnut water extract.
