CAJ | 학술논문

目的：探讨细胞因子IL‐17、IL‐22和IL‐10在过敏性哮喘患者外周血中的变化及临床意义。方法选取过敏性哮喘患者40例（哮喘组）和健康体检者30例（对照组），采用ELISA法检测外周血培养上清中IL‐17、IL‐22和IL‐10的表达；电化学发光免疫分析法检测血清中Ig E的水平，全自动血液分析仪检测全血嗜酸性粒细胞比率；IL‐17、IL‐22和IL‐10与哮喘严重程度进行Pearson相关性分析。结果哮喘组血清中IgE的水平及全血嗜酸性粒细胞比率高于对照组（P＜0．05）；哮喘组外周血IL‐17、IL‐22水平高于对照组，而IL‐10水平低于对照组（P＜0．05）；哮喘严重程度与IL‐17、IL‐22水平呈正相关（P＜0．01），而与IL‐10水平呈负相关（ P＜0．05）。结论 IL‐17、IL‐22和IL‐10在过敏性哮喘的发病机制中起重要作用，动态监测其变化有利于临床诊断及治疗。
목적：탐토세포인자IL‐17、IL‐22화IL‐10재과민성효천환자외주혈중적변화급림상의의。방법선취과민성효천환자40례（효천조）화건강체검자30례（대조조），채용ELISA법검측외주혈배양상청중IL‐17、IL‐22화IL‐10적표체；전화학발광면역분석법검측혈청중Ig E적수평，전자동혈액분석의검측전혈기산성립세포비솔；IL‐17、IL‐22화IL‐10여효천엄중정도진행Pearson상관성분석。결과효천조혈청중IgE적수평급전혈기산성립세포비솔고우대조조（P＜0．05）；효천조외주혈IL‐17、IL‐22수평고우대조조，이IL‐10수평저우대조조（P＜0．05）；효천엄중정도여IL‐17、IL‐22수평정정상관（P＜0．01），이여IL‐10수평정부상관（ P＜0．05）。결론 IL‐17、IL‐22화IL‐10재과민성효천적발병궤제중기중요작용，동태감측기변화유리우림상진단급치료。
Objective To observe the clinical change of cytokines expression in peripheral blood in patients with allergic asthma and its significance .Methods Forty patients with allergic asthma and thirty healthy controls were enrolled .The concentrations of IL‐17 ,IL‐22 and IL‐10 in peripheral blood were measured by ELISA ;the serum IgE levels were measured by electrochemical lumi‐nescence method ;The eosinophil proportion was detected by blood cell analyzer ;the IL‐17 ,IL‐22 ,IL‐10 and the severity of asthma were conducted Pearson correlation analysis .Results The level of serum IgE and eosinophil proportion was significantly higher in patients with allergic asthma compared with the controls (P<0 .05);compared with the control group ,the concentration of IL‐17 and IL‐22 in peripheral blood of patients with allergic asthma were increased ,but the expression of IL‐10 were decreased (P<0 .05);the level of IL‐17 and IL‐22 were positively correlated with severity of allergic asthma (P<0 .01) ,but the level of IL‐10 was negatively correlated with severity of allergic asthma (P<0 .05) .Conclusion The expression of IL‐17 ,IL‐22 and IL‐10 play an im‐portant role in the pathogenetic mechanism of allergic asthma ,and a dynamic monitoring could be helpful for the diagnoses and treatment of allergic asthma .