CAJ | 학술논문

目的：观察Slit2蛋白对大鼠胸主动脉血管平滑肌细胞（VSMCs）增殖迁移的影响。方法采用川北医学院第二临床医学院实验室培养的大鼠VSMCs ，实验分两部分，第一部分：Slit2单独作用，分为正常对照组和实验组（Slit2蛋白浓度分别为：50、75、100、125、150 ng／mL）；第二部分：Slit2与TNF‐α共同作用，分为正常对照组、阳性对照组（含TNF‐α10 ng／mL）和实验组（TNF‐α10 ng／mL＋Slit250 ng／mL、TNF‐α10 ng／mL＋Slit275 ng／mL、TNF‐α10 ng／mL＋Slit2100 ng／mL、TNF‐α10 ng／mL＋Slit2125 ng／mL、TNF‐α10 ng／mL＋Slit2150 ng／mL）。分别采用CCK‐8及transwell小室法检测各组细胞的增殖及迁移能力。结果第一部分：实验组与对照组OD值及迁移细胞数目比较差异均无统计学意义（P＝0．516，P＝0．52）。第二部分：正常对照组与阳性对照组比较细胞迁移数目差异有统计学意义（P＝0．00），实验组细胞迁移数较阳性对照组明显减少；CCK‐8结果显示实验组和阳性对照组与正常对照组OD值比较差异有统计学意义（P＜0．05），而实验组与阳性对照组OD值比较差异无统计学意义（P＝0．173）。结论 Slit2对VSMCs增殖无影响，但能抑制TNF‐α诱导的VSMCs迁移。
목적：관찰Slit2단백대대서흉주동맥혈관평활기세포（VSMCs）증식천이적영향。방법채용천북의학원제이림상의학원실험실배양적대서VSMCs ，실험분량부분，제일부분：Slit2단독작용，분위정상대조조화실험조（Slit2단백농도분별위：50、75、100、125、150 ng／mL）；제이부분：Slit2여TNF‐α공동작용，분위정상대조조、양성대조조（함TNF‐α10 ng／mL）화실험조（TNF‐α10 ng／mL＋Slit250 ng／mL、TNF‐α10 ng／mL＋Slit275 ng／mL、TNF‐α10 ng／mL＋Slit2100 ng／mL、TNF‐α10 ng／mL＋Slit2125 ng／mL、TNF‐α10 ng／mL＋Slit2150 ng／mL）。분별채용CCK‐8급transwell소실법검측각조세포적증식급천이능력。결과제일부분：실험조여대조조OD치급천이세포수목비교차이균무통계학의의（P＝0．516，P＝0．52）。제이부분：정상대조조여양성대조조비교세포천이수목차이유통계학의의（P＝0．00），실험조세포천이수교양성대조조명현감소；CCK‐8결과현시실험조화양성대조조여정상대조조OD치비교차이유통계학의의（P＜0．05），이실험조여양성대조조OD치비교차이무통계학의의（P＝0．173）。결론 Slit2대VSMCs증식무영향，단능억제TNF‐α유도적VSMCs천이。
Objective To observe the effects of Slit2 protein on the proliferation and migration of VSMCs .Methods The VSMCs was cultured in our laboratory .The experiment was divided into two parts ,part one:VSMCs were divided into normal con‐trol group and experimental groups(culture with 50 ,75 ,100 ,125 and 150 ng/mL Slit2 respectively);part two :VSMCs were divided into normal control group ,positive control group(culture with TNF‐α10 ng/mL) and experimental groups(culture with TNF‐α10 ng/mL+Slit2 50 ng/mL ,TNF‐α10 ng/mL+Slit2 75ng/mL ,TNF‐α10 ng/mL+ Slit2 100 ng/mL ,TNF‐α10 ng/mL+ Slit2 125 ng/mL and TNF‐α10 ng/mL+Slit2 150 ng/mL respectively) .To detect proliferation and migration of VSMCs by CCK‐8 and tran‐swell experiment .Results The difference of OD value and numbers of VSMCs has no statistical significance in the presence of Slit 2 (P=0 .516 ,P=0 .52) .The numbers of VSMCs has statistical significance between control and positive control groups (P=0 .00) . The numbers of VSMCs in experimental groups were fewer than positive control group (P<0 .05) ,whereas the difference of OD value still has no statistical significance between experimental and positive groups (P= 0 .173) .Conclusion Recombinant Slit2 could inhibits migration in VSMCs induced by TNF‐α,whereas it has no effect on proliferation of VSMCs .