重庆医学
重慶醫學
중경의학
CHONGQING MEDICAL JOURNAL
2015年
4期
459-461
,共3页
心肌梗死%血小板活化%强化他汀%炎症因子
心肌梗死%血小闆活化%彊化他汀%炎癥因子
심기경사%혈소판활화%강화타정%염증인자
myocardial infarction%platelet activation%intensive statin%inflammation factors
目的：探讨强化他汀对大鼠心肌梗死后24 h血小板活化及炎症因子的影响。方法70只SD大鼠分为SHAM 组、心肌梗死对照组、常规剂量组、强化他汀组、混合剂量组，每组14只。予以手术开胸结扎大鼠心脏左冠状动脉前降支制备心肌梗死动物模型（SHAM组只穿线不结扎）。术前常规剂量组、混合剂量组分别予以小剂量阿托伐他汀（10 mg · kg －1· d－1）灌胃2周；强化他汀组、混合剂量组术前12 h给予单剂阿托伐他汀50 mg／kg 灌胃；各组大鼠于术后24 h采血检测肿瘤坏死因子‐α（TNF‐α）、超敏C‐反应蛋白（hs‐CRP）、血小板活化因子‐1（PAC‐1）、CD62p。结果术后24 h急性炎症因子 TNF‐α、hs‐CRP水平心肌梗死对照组显著高于SHAM 组，血小板活化表面标志物PAC‐1、CD62p亦显著性高于SHAM 组（P＜0．05）；强化他汀组炎症因子水平，血小板活化标志物显著低于心肌梗死对照组（P＜0．05）；强化他汀组与混合剂量组比较，在炎症因子，血小板活化标志物水平差异无统计学意义（P＞0．05）；常规剂量他汀组在心肌梗死大鼠术后短期与心肌梗死对照组比较炎症因子，血小板活化标志物水平差异无统计学意义（P＞0．05）。结论术前使用强化阿托伐他汀能显著降低心肌梗死大鼠术后急性炎性反应，抑制血小板活化。
目的：探討彊化他汀對大鼠心肌梗死後24 h血小闆活化及炎癥因子的影響。方法70隻SD大鼠分為SHAM 組、心肌梗死對照組、常規劑量組、彊化他汀組、混閤劑量組，每組14隻。予以手術開胸結扎大鼠心髒左冠狀動脈前降支製備心肌梗死動物模型（SHAM組隻穿線不結扎）。術前常規劑量組、混閤劑量組分彆予以小劑量阿託伐他汀（10 mg · kg －1· d－1）灌胃2週；彊化他汀組、混閤劑量組術前12 h給予單劑阿託伐他汀50 mg／kg 灌胃；各組大鼠于術後24 h採血檢測腫瘤壞死因子‐α（TNF‐α）、超敏C‐反應蛋白（hs‐CRP）、血小闆活化因子‐1（PAC‐1）、CD62p。結果術後24 h急性炎癥因子 TNF‐α、hs‐CRP水平心肌梗死對照組顯著高于SHAM 組，血小闆活化錶麵標誌物PAC‐1、CD62p亦顯著性高于SHAM 組（P＜0．05）；彊化他汀組炎癥因子水平，血小闆活化標誌物顯著低于心肌梗死對照組（P＜0．05）；彊化他汀組與混閤劑量組比較，在炎癥因子，血小闆活化標誌物水平差異無統計學意義（P＞0．05）；常規劑量他汀組在心肌梗死大鼠術後短期與心肌梗死對照組比較炎癥因子，血小闆活化標誌物水平差異無統計學意義（P＞0．05）。結論術前使用彊化阿託伐他汀能顯著降低心肌梗死大鼠術後急性炎性反應，抑製血小闆活化。
목적：탐토강화타정대대서심기경사후24 h혈소판활화급염증인자적영향。방법70지SD대서분위SHAM 조、심기경사대조조、상규제량조、강화타정조、혼합제량조，매조14지。여이수술개흉결찰대서심장좌관상동맥전강지제비심기경사동물모형（SHAM조지천선불결찰）。술전상규제량조、혼합제량조분별여이소제량아탁벌타정（10 mg · kg －1· d－1）관위2주；강화타정조、혼합제량조술전12 h급여단제아탁벌타정50 mg／kg 관위；각조대서우술후24 h채혈검측종류배사인자‐α（TNF‐α）、초민C‐반응단백（hs‐CRP）、혈소판활화인자‐1（PAC‐1）、CD62p。결과술후24 h급성염증인자 TNF‐α、hs‐CRP수평심기경사대조조현저고우SHAM 조，혈소판활화표면표지물PAC‐1、CD62p역현저성고우SHAM 조（P＜0．05）；강화타정조염증인자수평，혈소판활화표지물현저저우심기경사대조조（P＜0．05）；강화타정조여혼합제량조비교，재염증인자，혈소판활화표지물수평차이무통계학의의（P＞0．05）；상규제량타정조재심기경사대서술후단기여심기경사대조조비교염증인자，혈소판활화표지물수평차이무통계학의의（P＞0．05）。결론술전사용강화아탁벌타정능현저강저심기경사대서술후급성염성반응，억제혈소판활화。
Objective To explore the effect of intensive statin on platelet activity and inflammation factors 24 h after rat myocar‐dial infarction .Methods Seventy Sprague‐Dawley rats were randomly divided into five groups (n= 14):Sham‐operated group (SHAM group);AMI group(control group) ,general group;intensive statin therapy group ;general and intensive statin therapy group;established AMI rat model by ligation of left anterior descending branch of coronary artery .The general group ,general and intensive statin therapy group was fed atorvastatin by 10 mg · kg -1 · d-1 with distilled water 2 mL by intragastric gavage daily for two weeks .The intensive statin therapy group ,general and intensive statin therapy group was fed atorvastatin by 50 mg/kg with distilled water 2 mL by intragastric gavage 12 h before surgery .Serum PAC‐1 ,CD62p ,TNF‐α,hs‐CRP was measured at the time of 24 h of postoperation .Results TNF‐α,hs‐CRP ,PAC‐1 and CD62p levels in control group were significantly higher than the SHAM group and intensive statin group 24 h after the LADS were ligated(P<0 .05);and the factors of intensive statin group were signifi‐cantly lower than that of control group(P<0 .05) .There was no significant difference between the intensive statin group and gener‐al‐intensive group in the concentration of serum TNF ,hs‐CRP and the relative expression of PAC‐1 and CD62p(P> 0 .05);and there was no significant difference between normal group and control group in all the four factors (P>0 .05) .Conclusion Intensive statin therapy before acute myocardial infarction could decrease the level of inflammation factors and inhibit platelet activity postop‐eration .
