CAJ | 학술논문

目的：研究人乳腺癌细胞微球体（MSs）的生物学特性，建立乳腺癌干细胞实验模型。方法无血清悬浮培养人乳腺癌MCF‐7细胞（MCF‐7组），并获取MSs（MSs组）。利用细胞划痕实验、transwell实验及动物成瘤实验检测MSs在细胞迁移、侵袭性生长及体外成瘤等方面的生物学特性。结果细胞划痕实验提示：MSs组划痕带可在48 h后愈合，而MCF‐7组划痕带在48 h后未能愈合；transwell实验提示MSs组中可见到（76．24±0．35）个细胞通过生物膜，而MCF‐7细胞组中为（17．38±0．18）个（P＜0．05）；动物成瘤实验提示：MSs在成瘤速度及移植瘤体积方面均强于MCF‐7细胞。结论 MSs具有极强的迁移、侵袭性生长及动物体内成瘤的能力，可作为实验模型应用于乳腺癌干细胞相关研究中。
목적：연구인유선암세포미구체（MSs）적생물학특성，건립유선암간세포실험모형。방법무혈청현부배양인유선암MCF‐7세포（MCF‐7조），병획취MSs（MSs조）。이용세포화흔실험、transwell실험급동물성류실험검측MSs재세포천이、침습성생장급체외성류등방면적생물학특성。결과세포화흔실험제시：MSs조화흔대가재48 h후유합，이MCF‐7조화흔대재48 h후미능유합；transwell실험제시MSs조중가견도（76．24±0．35）개세포통과생물막，이MCF‐7세포조중위（17．38±0．18）개（P＜0．05）；동물성류실험제시：MSs재성류속도급이식류체적방면균강우MCF‐7세포。결론 MSs구유겁강적천이、침습성생장급동물체내성류적능력，가작위실험모형응용우유선암간세포상관연구중。
Objective To study the biological characteristics of the human breast cancer cell mammospheres (MSs) ,and con‐struct breast cancer stem cell experiment model .Methods MCF‐7 cells were cultured in the serum‐free media supplemented with growth factors (the MCF‐7 group) ,and the MSs was collected (the MSs group) .The migration ,invasive and animal tumor forma‐tion abilities of MSs were detected by wound healing ,transwell invasive assay and animal tumor formation test .Results The wound line of MSs healed after 48 hours ,but the line of MCF‐7cells could not heal after 48 h .The number of the cells going through the membrane in MSs group was (76 .24 ± 0 .35) ,and the number in MCF‐7cells was (17 .38 ± 0 .18)(P<0 .05) .MSs had stronger ani‐mal tumor formation ability than MCF‐7 cells .Conclusion MSs have stronger abilities in migration ,invasive and animal tumor for‐mation ,and could be used in the studies of breast cancer stem cell as experimental model .