CAJ | 학술논문

通过对我市手足口病病毒种类进行初步筛查鉴定，建立逆转录环介导等温扩增技术( RT-LAMP)的研究平台，以评价LAMP技术在手足口病病毒毒检测方面是否具有优越性。通过采集临床确诊病人的咽拭子比较RT-PCR、 RT-LAMP三种检测方法来确定对于手足口病病毒最有优势的检测方法。 RT-PCR检出阳性率为70%， RT-LAMP的检出阳性率为65%。 RT-LAMP方法用于手足口病病毒的检测准确可行，简单快速，值得在基层医院推广。
통과대아시수족구병병독충류진행초보사사감정，건립역전록배개도등온확증기술( RT-LAMP)적연구평태，이평개LAMP기술재수족구병병독독검측방면시부구유우월성。통과채집림상학진병인적인식자비교RT-PCR、 RT-LAMP삼충검측방법래학정대우수족구병병독최유우세적검측방법。 RT-PCR검출양성솔위70%， RT-LAMP적검출양성솔위65%。 RT-LAMP방법용우수족구병병독적검측준학가행，간단쾌속，치득재기층의원추엄。
To screen and identify the isotype of virus related with Hand, Foot, and mouth disease, the reverse loop-mediated isothermal amplification ( RT-LAMP) research platform was built in order to evaluate the value of RT-LAMP.Throat swabs specimens with HFMD diagnosed by our hospital from 2011 to 2013 were selected. Virus was detected by using three methods:cytopathic effect ( CPE) , RT-PCR and RT-LAMP. CPE was not be obvious, the positive rate was 70% by RT-PCR, and the positive rate was 65% by RT-LAMP. It was feasible and accurate to detect virus related with HFMD by RT-LAMP, and the method was rapid and convenient, it is worthy of spreading to clinical application in primary hospital.