CAJ | 학술논문

目的：探讨刺五加(Aeanthopanax senticosus，AS)注射液预处理对大鼠肾缺血再灌注损伤(Ischemia reperfusion injury，IRI)的保护作用及其机制。方法于2013年12月至2014年5月，实验采用单动脉夹闭法构建大鼠急性肾IRI模型。选用36只健康雄性SD大鼠，按照随机数字法，随机分为六组，每组6只：(1)对照5 h组(Control 5 h组)；(2)对照10 h组(Control 10 h组)；(3)缺血再灌注损伤5 h组(IRI 5 h组)；(4)缺血再灌注损伤10 h组(IRI 10 h组)；(5)刺五加注射液预处理后肾缺血再灌注损伤5 h组(AS 5 h)；(6)刺五加注射液预处理后肾缺血再灌注损伤10 h组(AS 10 h)。Control组术中仅切除右肾；IRI组及AS组采用切除大鼠右肾，夹闭左侧肾动脉，45 min后解除夹闭。AS组于术前5 d每天给予1次及术前0.5 h给予1次刺五加注射液，按100 mg/kg腹腔注射；Control组及IRI组术前5 d及术前0.5 h给予腹腔注射和AS同等剂量的生理盐水。各组于相应的时间点采集下腔静脉血及左肾组织，然后处死各组实验大鼠，检测各组大鼠血清肌酐(Scr)、尿素氮(BUN)，肾组织超氧化物岐化酶(SOD)活性及丙二醛(MDA)含量，HE染色光镜下观察各组大鼠肾脏组织的病理变化。结果 AS预处理组肾组织病理变化轻于IRI组；与Control组相比较，IRI组的BUN、Scr水平升高(P<0.05)，肾组织中的SOD降低及MDA水平增加(P<0.05)。而AS预处理组的Scr、BUN和肾组织的MDA均比IRI组低(P<0.05)，而肾组织中的SOD水平升高(P<0.05)。结论 AS对肾脏缺血再灌注损伤具有保护作用，其机制可能是通过抗氧化作用实现的。
목적：탐토자오가(Aeanthopanax senticosus，AS)주사액예처리대대서신결혈재관주손상(Ischemia reperfusion injury，IRI)적보호작용급기궤제。방법우2013년12월지2014년5월，실험채용단동맥협폐법구건대서급성신IRI모형。선용36지건강웅성SD대서，안조수궤수자법，수궤분위륙조，매조6지：(1)대조5 h조(Control 5 h조)；(2)대조10 h조(Control 10 h조)；(3)결혈재관주손상5 h조(IRI 5 h조)；(4)결혈재관주손상10 h조(IRI 10 h조)；(5)자오가주사액예처리후신결혈재관주손상5 h조(AS 5 h)；(6)자오가주사액예처리후신결혈재관주손상10 h조(AS 10 h)。Control조술중부절제우신；IRI조급AS조채용절제대서우신，협폐좌측신동맥，45 min후해제협폐。AS조우술전5 d매천급여1차급술전0.5 h급여1차자오가주사액，안100 mg/kg복강주사；Control조급IRI조술전5 d급술전0.5 h급여복강주사화AS동등제량적생리염수。각조우상응적시간점채집하강정맥혈급좌신조직，연후처사각조실험대서，검측각조대서혈청기항(Scr)、뇨소담(BUN)，신조직초양화물기화매(SOD)활성급병이철(MDA)함량，HE염색광경하관찰각조대서신장조직적병리변화。결과 AS예처리조신조직병리변화경우IRI조；여Control조상비교，IRI조적BUN、Scr수평승고(P<0.05)，신조직중적SOD강저급MDA수평증가(P<0.05)。이AS예처리조적Scr、BUN화신조직적MDA균비IRI조저(P<0.05)，이신조직중적SOD수평승고(P<0.05)。결론 AS대신장결혈재관주손상구유보호작용，기궤제가능시통과항양화작용실현적。
Objective To observe the protective effect of Aeanthopanax senticosus (AS) and discuss its pos-sible mechanism on rats with acute renal ischemic reperfusion injury (IRI). Methods Rats with acute renal ischemia reperfusion injury model were constructed by single-artery occlusion. Thirty-six healthy male SD rats were selected and randomly divided into six groups:Control 5 h group, Control 10 h group, IRI 5 h group, IRI 10 h group, AS 5 h group, AS 10 h group. In Control group, right kidney was removed. In IRI and AS group, right kidney was removed, and the left renal was occlusived for 45 minutes. AS 5 h group, AS group rats were injected 100 mg/kg AS intraperito-neally once per day in the 5 days before surgery and 30 minutes before surgery. Control group and IRI group rats were injected saline at the equivalent dose at the same time. Venous blood and left kidney tissue were taken at correspond-ing time point (5 h and 10 h) in each group. The levels of Scr and BUN were measured, as well as SOD and MDA ac-tivity of kidney tissue. Pathological changes of renal tissue were observed by H-E staining technique. Results Patho-logical changes of renal tissue were lighter in AS group than IRI group. Compared with Control group, BUN, Scr lev-els in IRI were significantly increased (P<0.05), with SOD decreased and MDA increased (P<0.05). Compared with IRI group, Scr, BUN, MDA in AS group were significantly lower (P<0.05). Conclusion Aeanthopanax senticosus could protect the kidney against renal ischemia reperfusion injury, which might be realized by its antioxidant.