现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
4期
517-519
,共3页
杨松%熊依军%邹韬%杨兴明%黄文岳
楊鬆%熊依軍%鄒韜%楊興明%黃文嶽
양송%웅의군%추도%양흥명%황문악
供血者%血清学%四川%D变异体%DEL型%不规则抗体
供血者%血清學%四川%D變異體%DEL型%不規則抗體
공혈자%혈청학%사천%D변이체%DEL형%불규칙항체
Blood donors%Serology%Sichuan%D variants%DEL type%Irregular antibody
目的:研究四川省南充地区初检为Rh阴性无偿献血者中D变异体血清学表型。方法对433份初检为Rh阴性的血样(来自于2010年4月至2014年9月四川省南充地区无亲缘关系的154392名汉族无偿献血者)用间接抗球蛋白试验鉴定D变异体,再用吸收放散试验从间接抗球蛋白试验阴性的血样检出DEL型,对所有血样进行不规则抗体筛选鉴定。结果433份送检标本中共确认Rh阴性402例(92.8%,402/433),检出23例D变异体(5.3%,23/433),其中DvccEe 8例(34.8%,8/23),DvCcee和DvCcEe各6例(分别为26.1%,6/23),DvCCee 3例(13.0%,3/23);检出DEL型3种表型105例,其中DelCcee 87例(82.9%,87/105),DelCCee 16例(15.2%,16/105),DelCcEe 2例(1.9%,2/105),均与C抗原相关;在D变异体和DEL型献血者血浆标本中未检出不规则抗体。结论通过该次调查,掌握了四川省南充地区初检为Rh阴性献血者D变异体血清学表型,对于指导临床输血及预防新生儿溶血病等具有重要意义。
目的:研究四川省南充地區初檢為Rh陰性無償獻血者中D變異體血清學錶型。方法對433份初檢為Rh陰性的血樣(來自于2010年4月至2014年9月四川省南充地區無親緣關繫的154392名漢族無償獻血者)用間接抗毬蛋白試驗鑒定D變異體,再用吸收放散試驗從間接抗毬蛋白試驗陰性的血樣檢齣DEL型,對所有血樣進行不規則抗體篩選鑒定。結果433份送檢標本中共確認Rh陰性402例(92.8%,402/433),檢齣23例D變異體(5.3%,23/433),其中DvccEe 8例(34.8%,8/23),DvCcee和DvCcEe各6例(分彆為26.1%,6/23),DvCCee 3例(13.0%,3/23);檢齣DEL型3種錶型105例,其中DelCcee 87例(82.9%,87/105),DelCCee 16例(15.2%,16/105),DelCcEe 2例(1.9%,2/105),均與C抗原相關;在D變異體和DEL型獻血者血漿標本中未檢齣不規則抗體。結論通過該次調查,掌握瞭四川省南充地區初檢為Rh陰性獻血者D變異體血清學錶型,對于指導臨床輸血及預防新生兒溶血病等具有重要意義。
목적:연구사천성남충지구초검위Rh음성무상헌혈자중D변이체혈청학표형。방법대433빈초검위Rh음성적혈양(래자우2010년4월지2014년9월사천성남충지구무친연관계적154392명한족무상헌혈자)용간접항구단백시험감정D변이체,재용흡수방산시험종간접항구단백시험음성적혈양검출DEL형,대소유혈양진행불규칙항체사선감정。결과433빈송검표본중공학인Rh음성402례(92.8%,402/433),검출23례D변이체(5.3%,23/433),기중DvccEe 8례(34.8%,8/23),DvCcee화DvCcEe각6례(분별위26.1%,6/23),DvCCee 3례(13.0%,3/23);검출DEL형3충표형105례,기중DelCcee 87례(82.9%,87/105),DelCCee 16례(15.2%,16/105),DelCcEe 2례(1.9%,2/105),균여C항원상관;재D변이체화DEL형헌혈자혈장표본중미검출불규칙항체。결론통과해차조사,장악료사천성남충지구초검위Rh음성헌혈자D변이체혈청학표형,대우지도림상수혈급예방신생인용혈병등구유중요의의。
Objective To study the serological phenotype of D variants in volunteer blood donors initially-diagnosed Rh negative in Nanchong region of Sichuan province. Methods A total of 433 blood samples from 154 392 unrelated volunteer blood donors with Han nationality in Nanchong district of Sichuan province initially diagnosed as Rh negative were identified by indirect antiglobulin test for D variants ,and the negative results were subsequently detected by absorption-elution testing for DEL type from indirect antiglobulin testing. In addition ,all of the blood samples were screened and identified for irregular antibodies. Results Among 433 samples,402 cases(92.8%,402/433) were confirmed Rh negative and 23 cases(5.3%,23/433) were de-tected D variants,including DvccEe for 8 cases(34.8%,8/23),DvCcee for 6 cases(26.1%,6/23) and DvCCee for 3 cases(13.0%, 3/23);A total of 3 DEL phenotypes for 105 cases were found,including DelCcee for 87 cases(82.9%,87/105),DelCCee for 16 cases (15.2%,16/105),and DelCcEe for 2 cases(1.9%,2/105),all of which were associated with C antigen;Furthermore,irregular antibodies failed to be detected in plasma samples of donors with D variants and DEL type. Conclusion In the survey,serologi-cal phenotypes of D variant in volunteer blood donors initially-diagnosed Rh negative in Nanchong district of Sichuan province are learned,which has an important significance to guide the clinical blood transfusion and prevent hemolytic diseases of the newborns.