现代医药卫生
現代醫藥衛生
현대의약위생
MODERN MEDICINE HEALTH
2015年
4期
481-484
,共4页
黄芪多糖%顺铂%半胱氨酸天冬氨酸蛋白酶3%人肺癌A549细胞%Bcl相关X蛋白%B细胞淋巴瘤-白血病2
黃芪多糖%順鉑%半胱氨痠天鼕氨痠蛋白酶3%人肺癌A549細胞%Bcl相關X蛋白%B細胞淋巴瘤-白血病2
황기다당%순박%반광안산천동안산단백매3%인폐암A549세포%Bcl상관X단백%B세포림파류-백혈병2
Astragalan%Cisplatin%Caspase 3%human lung cancer A549 cells%Bcl associated X protein%B cell lymphoma/leukemia-2
目的：观察黄芪多糖与顺铂对人肺癌A549细胞增殖、细胞周期、诱导凋亡的影响，并对其作用机制进行初步探讨。方法采用四甲基偶氮唑盐法检测黄芪多糖、顺铂及两药联合对细胞增殖的影响；用流式细胞仪分析对细胞周期及凋亡率的影响；用反转录聚合酶链反应（RT-PCR）和Western blotting法检测对人肺癌A549细胞B细胞淋巴瘤-白血病2（Bcl-2）、Bcl相关X蛋白（Bax）和半胱氨酸天冬氨酸蛋白酶3（Caspase-3）基因表达水平的影响。结果黄芪多糖、顺铂及两药联合对人肺癌A549细胞的增殖抑制作用呈时间-浓度依赖关系；与阴性对照组比较，黄芪多糖作用后可出现G1期细胞阻滞，顺铂作用后出现S期细胞阻滞，两药联合出现G1、S期细胞阻滞，差异均有统计学意义（P<0.01）。两药联合较阴性对照组及单用黄芪多糖、顺铂明显上调Bax、Caspase-3 mRNA及蛋白的表达，下调Bcl-2 mRNA及蛋白的表达，差异均有统计学意义（P<0.01）。结论黄芪多糖可协同顺铂增强对人肺癌A549细胞的促凋亡作用，其作用机制与上调Bax、Caspase-3的表达、下调Bcl-2的表达有关。
目的：觀察黃芪多糖與順鉑對人肺癌A549細胞增殖、細胞週期、誘導凋亡的影響，併對其作用機製進行初步探討。方法採用四甲基偶氮唑鹽法檢測黃芪多糖、順鉑及兩藥聯閤對細胞增殖的影響；用流式細胞儀分析對細胞週期及凋亡率的影響；用反轉錄聚閤酶鏈反應（RT-PCR）和Western blotting法檢測對人肺癌A549細胞B細胞淋巴瘤-白血病2（Bcl-2）、Bcl相關X蛋白（Bax）和半胱氨痠天鼕氨痠蛋白酶3（Caspase-3）基因錶達水平的影響。結果黃芪多糖、順鉑及兩藥聯閤對人肺癌A549細胞的增殖抑製作用呈時間-濃度依賴關繫；與陰性對照組比較，黃芪多糖作用後可齣現G1期細胞阻滯，順鉑作用後齣現S期細胞阻滯，兩藥聯閤齣現G1、S期細胞阻滯，差異均有統計學意義（P<0.01）。兩藥聯閤較陰性對照組及單用黃芪多糖、順鉑明顯上調Bax、Caspase-3 mRNA及蛋白的錶達，下調Bcl-2 mRNA及蛋白的錶達，差異均有統計學意義（P<0.01）。結論黃芪多糖可協同順鉑增彊對人肺癌A549細胞的促凋亡作用，其作用機製與上調Bax、Caspase-3的錶達、下調Bcl-2的錶達有關。
목적：관찰황기다당여순박대인폐암A549세포증식、세포주기、유도조망적영향，병대기작용궤제진행초보탐토。방법채용사갑기우담서염법검측황기다당、순박급량약연합대세포증식적영향；용류식세포의분석대세포주기급조망솔적영향；용반전록취합매련반응（RT-PCR）화Western blotting법검측대인폐암A549세포B세포림파류-백혈병2（Bcl-2）、Bcl상관X단백（Bax）화반광안산천동안산단백매3（Caspase-3）기인표체수평적영향。결과황기다당、순박급량약연합대인폐암A549세포적증식억제작용정시간-농도의뢰관계；여음성대조조비교，황기다당작용후가출현G1기세포조체，순박작용후출현S기세포조체，량약연합출현G1、S기세포조체，차이균유통계학의의（P<0.01）。량약연합교음성대조조급단용황기다당、순박명현상조Bax、Caspase-3 mRNA급단백적표체，하조Bcl-2 mRNA급단백적표체，차이균유통계학의의（P<0.01）。결론황기다당가협동순박증강대인폐암A549세포적촉조망작용，기작용궤제여상조Bax、Caspase-3적표체、하조Bcl-2적표체유관。
Objective To observe the inhibitory effects of astragalus polysaccharides and cisplatin on the cell prolifera-tion,cycle,apoptosis induction of human lung cancer A549 cells,and initially discuss its action mechanism. Methods The ef-fects on cell proliferation of astragalus polysaccharides ,cisplatin and their combinations was detected by MTT assay. It analyzed the impact of cell cycle and apoptosis rate by flow cytometry. The A549 human lung cancer cells Bax ,Bcl-2 and Caspase-3 gene expression levels by reverse transcription polymerase chain reaction (RT-PCR) and Western blotting. Results Astragalus polysac-charide,cisplatin and two-drug combination on inhibition of proliferation of human lung cancer A549 cells had a time-concentra-tion-dependent proliferation. Compared with the negative control group ,the cell cycle blockage in G1 phase after astragalus polysaccharide action while the cell cycle blockage in S phase after cisplatin action and the application of two-drug combination appeared cell cycle blockage by flow cytometry both in G1 and S phases(P<0.01). The expression of Bax,Caspase-3 mRNA and pro-tein were upgraded in the expression of the two-drug combination group and lowered in Bcl-2 mRNA protein compared to the nega-tive control and monotherapy group. The difference had statistical significance (P<0.05). Conclusion The astragalus polysaccharide combined with cisplatin may strengthen human lung cancer A549 cells to enhance the pro-apoptosis,whose mechanism is associ-ated with upgrading the expression of Bax ,Caspase-3 and lowering the expression of Bcl-2mRNA.
