CAJ | 학술논문

目的：统计结直肠癌中 CCNB2的表达情况，分析结直肠癌中 CCNB2阳性表达的临床意义。方法随机选取2010年6月～2013年11月份我院在手术治疗中留取的结直肠癌病标本57例和癌旁正常组织标本49例，用免疫组化法检测 CCNB2蛋白表达，用实时定量 PCR法检测 CCNB2 mRNA 表达。结果 CCNB2蛋白阳性表达主要在细胞质，偶见于细胞核。结直肠癌组织中 CCNB2 mRNA 相对表达量（36.52±8.82）和 CCNB2蛋白阳性表达率（86.21%）均显著高于癌旁正常组织，P<0.05，差异具有显著性。结论 CCNB2在结直肠癌组织呈高表达，有望作为诊断和预后的参考指标。
목적：통계결직장암중 CCNB2적표체정황，분석결직장암중 CCNB2양성표체적림상의의。방법수궤선취2010년6월～2013년11월빈아원재수술치료중류취적결직장암병표본57례화암방정상조직표본49례，용면역조화법검측 CCNB2단백표체，용실시정량 PCR법검측 CCNB2 mRNA 표체。결과 CCNB2단백양성표체주요재세포질，우견우세포핵。결직장암조직중 CCNB2 mRNA 상대표체량（36.52±8.82）화 CCNB2단백양성표체솔（86.21%）균현저고우암방정상조직，P<0.05，차이구유현저성。결론 CCNB2재결직장암조직정고표체，유망작위진단화예후적삼고지표。
Objective To sum up the expressions of CCNB2 in colorectal cancer and to analyze its clinical significance of positive expression. Methods From June 2010 to November 2013,57 specimens of colorectal cancer were taken during the surgery and 49 specimens of adjacent normal tissue were taken. They were randomly selected as the objects in our hospital. CCNB2 protein expression was detected by immunohistochemical method CCNB2mRNA expression was detected by real-time quantitative PCR method. Results The positive expression of CCNB2 protein was mainly in the cytoplasm and occasional y in the nucleus. The relative expression value of CCNB2mRNA in colorectal cancer tissues was (36.52±8.82) and CCNB2 protein positive expression rate was (86.21%). They were both significantly higher than that of adjacent normal tissues,P<0.05,the difference was significant. Conclusion In colorectal cancer tissues, CCNB2 showed high expressions, it is expected to become the early evaluation index of prognosis.