CAJ | 학술논문

为提高鱼腥草产业化的经济效益，获得含量较高的鱼腥草黄酮，并克服传统提取法效率低、溶剂消耗多、耗时长等问题，试验以黄酮含量为检测指标，考察乙醇浓度、液固比和提取时间对超声提取鱼腥草黄酮得率的影响。在单因素试验的基础上，通过 Box-Behnken 响应面分析法进行三因素三水平的试验设计。结果表明：提取鱼腥草黄酮的最佳工艺为乙醇浓度74．67％，超声时间69．88 min，液固比20．20∶1（mL／g），该条件下，预测鱼腥草黄酮得率为2．654％，实际得率为2．663％，与预测值相差接近，说明该模型合理有效。
위제고어성초산업화적경제효익，획득함량교고적어성초황동，병극복전통제취법효솔저、용제소모다、모시장등문제，시험이황동함량위검측지표，고찰을순농도、액고비화제취시간대초성제취어성초황동득솔적영향。재단인소시험적기출상，통과 Box-Behnken 향응면분석법진행삼인소삼수평적시험설계。결과표명：제취어성초황동적최가공예위을순농도74．67％，초성시간69．88 min，액고비20．20∶1（mL／g），해조건하，예측어성초황동득솔위2．654％，실제득솔위2．663％，여예측치상차접근，설명해모형합리유효。
The optimum conditions of ultrasonic extraction of total flavonoids from H .cordata was studied,to enhance the economical benefits,gain higher flavonoids content and overcome the shortcomings in traditional methods,such as inefficiency,solvent consumption and long time.The influence of ethanol concentration,extracting time and solid-liquid ratio was investigated by Box-Behnken response surface design.Results:The optimum conditions were as follows,ethanol concentration 74.67%,ultrasound time 69.88 min,liquid-solid ratio 20.20∶1(mL/g),under these conditions the predicted yield was 2.654%. The actual yield was 2.663%,which was slightly higher than the predicted yield,all the results showed that the model is reasonable and effective.