CAJ | 학술논문

目的：探讨宫颈鳞癌（cervical squamous cell carcinomas，CSCC）组织中表皮生长因子受体（EGFR）、Rab5a、Rab21蛋白表达及其与临床特征及近期疗效的关系，评估 EGFR、Rab5a、Rab21蛋白在 CSCC 进展、转移及近期疗效中的价值。方法收集60例 CSCC 组织标本，采用免疫组织化学法检测 EGFR、Rab5a、Rab21蛋白表达情况。采用卡方或确切概率法、非参数 Spearman 等级相关、多因素 Logistic 回归分析法分析其与临床特征及近期疗效的相关性。结果EGFR、Rab5a、Rab21蛋白阳性表达率分别为70．0％、73．3％、55．0％，EGFR、Rab5a、Rab21蛋白表达均与肿瘤分化程度、淋巴结转移有关（P ＜0．05），EGFR 蛋白表达与临床分期有关（P ＝0．005），Rab21蛋白表达与肿瘤直径有关（P ＝0．044）；Spearman 等级相关分析显示：EGFR 与 Rab5a 蛋白表达水平呈正相关（r ＝0．428，P ＝0．001），EGFR 与 Rab21蛋白表达水平呈负相关（r ＝－0．300，P ＝0．020）；EGFR、Rab5a、Rab21蛋白表达均不是影响近期疗效的独立因素（P ＞0．05），EGFR、Rab5a、Rab21蛋白表达对客观有效率 RR 无明显影响（P＞0．05）。结论EGFR 蛋白表达可能为 CSCC 高危进展的参考指标；Rab5a 在 EGFR 介导的信号传导通路中可能发挥正性调节作用；Rab21在 EGFR 介导的信号传导通路中可能发挥负性调节作用。
목적：탐토궁경린암（cervical squamous cell carcinomas，CSCC）조직중표피생장인자수체（EGFR）、Rab5a、Rab21단백표체급기여림상특정급근기료효적관계，평고 EGFR、Rab5a、Rab21단백재 CSCC 진전、전이급근기료효중적개치。방법수집60례 CSCC 조직표본，채용면역조직화학법검측 EGFR、Rab5a、Rab21단백표체정황。채용잡방혹학절개솔법、비삼수 Spearman 등급상관、다인소 Logistic 회귀분석법분석기여림상특정급근기료효적상관성。결과EGFR、Rab5a、Rab21단백양성표체솔분별위70．0％、73．3％、55．0％，EGFR、Rab5a、Rab21단백표체균여종류분화정도、림파결전이유관（P ＜0．05），EGFR 단백표체여림상분기유관（P ＝0．005），Rab21단백표체여종류직경유관（P ＝0．044）；Spearman 등급상관분석현시：EGFR 여 Rab5a 단백표체수평정정상관（r ＝0．428，P ＝0．001），EGFR 여 Rab21단백표체수평정부상관（r ＝－0．300，P ＝0．020）；EGFR、Rab5a、Rab21단백표체균불시영향근기료효적독립인소（P ＞0．05），EGFR、Rab5a、Rab21단백표체대객관유효솔 RR 무명현영향（P＞0．05）。결론EGFR 단백표체가능위 CSCC 고위진전적삼고지표；Rab5a 재 EGFR 개도적신호전도통로중가능발휘정성조절작용；Rab21재 EGFR 개도적신호전도통로중가능발휘부성조절작용。
Objective To investigate the relationship between EGFR,Rab5a,Rab21 protein expression and clinical features and the short-term efficacy in cervical squamous cell carcinoma.Methods Immunohisto-chemistry method was used to detect EGFR,Rab5a,Rab21 protein expression levels in 60 cases of CSCC. Chi-Square,Spearman rank correlation,Logistic regression analysis method were used.Results The pro-tein expression rates of EGFR,Rab5a,Rab21 were 70.0%,73.3%,55.0%,EGFR,Rab5a,Rab21 protein expression were correlated with lymph node metastasis and tumor differentiation (P <0.05);EG-FR protein expression was correlated with clinical stage (P =0.005);Rab21 protein expression was corre-lated with the diameter of tumor (P =0.044);Spearman rank correlation analysis showed that EGFR was positively correlated with Rab5a (r =0.428,P =0.001)and EGFR was negatively correlated with Rab21 expression (r =-0.300,P =0.020).EGFR,Rab5a and Rab21 protein expression were not the independ-ent factors of the short-term efficacy in CSCC (P >0.05);EGFR,Rab5a and Rab21 protein expression had no significant effect on objective efficiency (P >0.05).Conclusion EGFR protein expression may be the reference index of progression of CSCC;Rab5a may play a positive role in the regulation of EGFR-me-diated signal transduction pathway while Rab21 may play a negative role.