CAJ | 학술논문

目的：探讨肥大细胞糜蛋白酶在人瘢痕疙瘩组织中的表达活性。方法采用免疫组织化学法检测瘢痕疙瘩组织和正常皮肤中肥大细胞的数量和糜蛋白酶的表达，并以 RT-PCR 和放免法检测瘢痕疙瘩和正常皮肤组织中糜蛋白酶 mRNA 表达活性变化。结果瘢痕疙瘩组织中肥大细胞数量与糜蛋白酶阳性表达评分为（10±0．25）个／HP、（6．2±0．23）mm2，明显高于正常皮肤组织的（4±0．22）个／HP、（2．5±0．12）mm2（P ＜0．01）；RT-PCR结果显示瘢痕疙瘩组织中肥大细胞 mRNA 和糜蛋白酶 mRNA 表达水平较正常皮肤组织明显增高。结论肥大细胞糜蛋白酶存在于瘢痕疙瘩中且活性表达明显高于正常皮肤组织。
목적：탐토비대세포미단백매재인반흔흘탑조직중적표체활성。방법채용면역조직화학법검측반흔흘탑조직화정상피부중비대세포적수량화미단백매적표체，병이 RT-PCR 화방면법검측반흔흘탑화정상피부조직중미단백매 mRNA 표체활성변화。결과반흔흘탑조직중비대세포수량여미단백매양성표체평분위（10±0．25）개／HP、（6．2±0．23）mm2，명현고우정상피부조직적（4±0．22）개／HP、（2．5±0．12）mm2（P ＜0．01）；RT-PCR결과현시반흔흘탑조직중비대세포 mRNA 화미단백매 mRNA 표체수평교정상피부조직명현증고。결론비대세포미단백매존재우반흔흘탑중차활성표체명현고우정상피부조직。
Objective To explore the expressed activity of mast cells chymase exist in human keloid tissues. Methods The number of mast cell and chymase expression in keloid and normal skin were assessed by im-munohistochemical methods.Quantitative real-time PCR and Radio immunity method were conducted to measure the change of mRNA expressed activity of chymase in keloid and normal skin tissues.Results Com-pared with normal skin tissue,the number of mast cells and chymase expression were higher in keloid tissues [(10 ±0.25)/hp vs (4±0.22)/hp,and (6.2±0.23)mm2 vs (2.5±0.12)mm2 ,P <0.01)].The results of RT-PCR showed mast cell mRNA expression levels and chymase mRNA level were also significantly higher in keloid tissues than those of normal skin tissues (P <0.05).Conclusion The chymase of mast cells exists in keloids and the expressed activity of chymase is statistically higher than those of normal skin tissues.