CAJ | 학술논문

目的：以色素色价为指标，研究从黑桑中提取色素的较佳提取工艺。方法在单因素实验基础上，采用 L9（34）正交试验设计安排实验，采用紫外光谱法测定黑桑中色素的色价。选用乙醇浓度、提取时间、提取温度及提取 pH 值作为研究因素，以提取液中色素的色价为考察指标。结果各因素对色素提取率的影响程度大小顺序为：乙醇浓度＞提取温度＞提取 pH＞提取时间，乙醇浓度对色素提取的影响具有显著性，而提取时间、提取温度及提取 pH 值的影响不显著。经 L9（34）正交试验和方差分析，确定从黑桑中提取色素较佳工艺条件为：乙醇浓度为60％，温度为25℃，时间为6 h，pH 值为6。结论优化工艺合理可行，提取效率高，稳定成本低，重现性好，适合于大规模生产。
목적：이색소색개위지표，연구종흑상중제취색소적교가제취공예。방법재단인소실험기출상，채용 L9（34）정교시험설계안배실험，채용자외광보법측정흑상중색소적색개。선용을순농도、제취시간、제취온도급제취 pH 치작위연구인소，이제취액중색소적색개위고찰지표。결과각인소대색소제취솔적영향정도대소순서위：을순농도＞제취온도＞제취 pH＞제취시간，을순농도대색소제취적영향구유현저성，이제취시간、제취온도급제취 pH 치적영향불현저。경 L9（34）정교시험화방차분석，학정종흑상중제취색소교가공예조건위：을순농도위60％，온도위25℃，시간위6 h，pH 치위6。결론우화공예합리가행，제취효솔고，은정성본저，중현성호，괄합우대규모생산。
Objective To study the optimal technique of pigment extraction fromMorus nigra Linn,and to mensurate its colored value.Methods On the basis of single factor experiments,L9 (3 4 )orthogonal exper-iments were performed.The ultraviolet spectrophotometry method was used for mensurate pigment colored value fromM .nigra Linn.The ethanol concentration,extraction time,extraction temperature and pH value of extraction were studied with the colored value in content as the index.Result The influence order was as follows: ethanol concentration > extraction temperature > pH value of extraction >extraction time.The ethanol concentration had a significant effect on pigment extraction,while extraction time,extraction temperature and pH value of extraction had mot.The optimal conditions were as follows:60% of ethanol concentration,25℃ of extraction temperature,6 h of extraction time and 6 of extraction pH value.Conclusion The optimization process is reasonable and feasible.The method developed here is efficient,stable,repeatable and with lower cost.