北方药学
北方藥學
북방약학
JOURNAL OF NORTH PHARMACY
2015年
2期
1-1,2
,共2页
半枝莲总黄酮%脂质体%制备方法%包封率
半枝蓮總黃酮%脂質體%製備方法%包封率
반지련총황동%지질체%제비방법%포봉솔
Sculellaria barbata flavones%Liposomes%Preparation methods%The encapsulation rate
目的：探讨半枝莲总黄酮脂质体的制备，建立半枝莲总黄酮含量测定方法，并测定其包封率。方法：采用薄膜分散法来制备半枝莲总黄酮脂质体，采用超速离心法分离脂质体及游离药物，采用紫外分光光度法（UV）测定半枝莲总黄酮含量，并计算其包封率。结果：薄膜分散法制备的半枝莲总黄酮脂质体颜色为深褐色，呈圆形或类圆形，平均粒径为0.54μm，分布均匀，脂质体的平均包封率为94.38%，符合2010版《中国药典》的要求。结论：薄膜分散法可以简单快速地制备半枝莲总黄酮脂质体，操作流程掌握容易，制备的脂质体形态较好、包封率较高；超速离心法与UV结合可以准确、可靠地测定半枝莲总黄酮脂质体的包封率。
目的：探討半枝蓮總黃酮脂質體的製備，建立半枝蓮總黃酮含量測定方法，併測定其包封率。方法：採用薄膜分散法來製備半枝蓮總黃酮脂質體，採用超速離心法分離脂質體及遊離藥物，採用紫外分光光度法（UV）測定半枝蓮總黃酮含量，併計算其包封率。結果：薄膜分散法製備的半枝蓮總黃酮脂質體顏色為深褐色，呈圓形或類圓形，平均粒徑為0.54μm，分佈均勻，脂質體的平均包封率為94.38%，符閤2010版《中國藥典》的要求。結論：薄膜分散法可以簡單快速地製備半枝蓮總黃酮脂質體，操作流程掌握容易，製備的脂質體形態較好、包封率較高；超速離心法與UV結閤可以準確、可靠地測定半枝蓮總黃酮脂質體的包封率。
목적：탐토반지련총황동지질체적제비，건립반지련총황동함량측정방법，병측정기포봉솔。방법：채용박막분산법래제비반지련총황동지질체，채용초속리심법분리지질체급유리약물，채용자외분광광도법（UV）측정반지련총황동함량，병계산기포봉솔。결과：박막분산법제비적반지련총황동지질체안색위심갈색，정원형혹류원형，평균립경위0.54μm，분포균균，지질체적평균포봉솔위94.38%，부합2010판《중국약전》적요구。결론：박막분산법가이간단쾌속지제비반지련총황동지질체，조작류정장악용역，제비적지질체형태교호、포봉솔교고；초속리심법여UV결합가이준학、가고지측정반지련총황동지질체적포봉솔。
Objective:TO discuss how to prepare the liposome of Sculellaria barbata flavones,establish the method of total flavonoids determination,and determate its encapsulation rate.Methods:By using the film dispersion method to prepare the liposome of Sculellaria barbata flavones,and by using the ultracentrifugation to liposome and free drugs, and by using ultraviolet spectrophotometry (UV) to determinate the total content of flavonoids, and determate its encapsulation rate.Results:The Liposome color is dark brown,round or class round,the average particle size was 0.54 microns, uniform distribution,and the average of liposome encapsulation rate is 94.38%, it is in line with the requirements of “Chinese pharmacopoeia”2010 version.Conclusion:The film dispersion method is easy and simple to preparate the Sculellaria barbata flavones Liposome,the operation is easy to grasp,the morphology of liposomes prepared well and high encapsulation rate;The Ultracentrifugation method combined with UV can be accurate, reliable to determinate of the encapsulation rate.
