CAJ | 학술논문

为探明普安银鲫雌鱼不同发育时期与脂代谢相关的脂肪酸合成酶（FAS）、乙酰辅酶 A 羧化酶（ACC）浓度及相对表达量的变化，以了解胚胎早期在脂质合成代谢方面的特点和规律性，采用酶联免疫分析法和实时荧光定量 PCR 法，测定成熟卵、受精卵、囊胚期（卵裂期胚胎）的 FAS 和 ACC 代谢水平及基因表达情况。结果表明：普安银鲫雌鱼成熟卵、受精卵和囊胚期均存在 FAS 和 ACC，FAS 含量在囊胚期显著升高（P ＜0．05），达到（2．48±0．31）nmol／L；ACC 含量上升但差异不显著（P ＞0．05）。普安银鲫成熟卵、受精卵和囊胚期中均存在 FAS 和 ACC 基因表达，相对表达量逐渐增高，但差异均不显著（P ＞0．05）。
위탐명보안은즉자어불동발육시기여지대사상관적지방산합성매（FAS）、을선보매 A 최화매（ACC）농도급상대표체량적변화，이료해배태조기재지질합성대사방면적특점화규률성，채용매련면역분석법화실시형광정량 PCR 법，측정성숙란、수정란、낭배기（란렬기배태）적 FAS 화 ACC 대사수평급기인표체정황。결과표명：보안은즉자어성숙란、수정란화낭배기균존재 FAS 화 ACC，FAS 함량재낭배기현저승고（P ＜0．05），체도（2．48±0．31）nmol／L；ACC 함량상승단차이불현저（P ＞0．05）。보안은즉성숙란、수정란화낭배기중균존재 FAS 화 ACC 기인표체，상대표체량축점증고，단차이균불현저（P ＞0．05）。
To explore the variation of concentration and relative transcript level of fatty acid synthetase (FAS)and Acetyl CoA carboxylase (ACC)in different stages of C.auratus,so as to know the features and regularity of lipid anabolism in early stage of embryo,both the enzyme concentration and mRNA expression level of FAS and ACC were determined in mature egg and embryo after fertilization by ELISA and real-time PCR.Results:Enzyme concentration existed in mature egg,fertilized egg and blastula stage embryo.FAS enzyme concentration increased to (2.48 ± 0.31 )nmol/L (P < 0.05 ),ACC enzyme concentration increased gradually (P >0.05).FAS and ACC gene expressed in mature egg,fertilized egg and blastula stage embryo,relative transcript level increased gradually(P >0.05).