CAJ | 학술논문

为研究黑曲霉菌株（Aspergillus niger ）XZ-3S 曲盘发酵木聚糖酶条件及所产粗酶的性质，采用曲盘（大小φ20 cm×4 cm）进行固态发酵条件研究，并对所产粗酶进行酶学性质测定。结果表明：在培养基干料100 g、初始料水比1∶1．7、初始 pH 自然状态、相对干物料接种量10％、中间定时翻曲补水和28℃连续培养60 h 条件下，酶活力最高可达24380 IU／g 干曲；粗酶最适反应 pH5．0，最适温度50℃；温度低于50℃、pH3．0～8．0的条件下酶较稳定；EDTA（活性物质）、Cu2＋、Co2＋对酶有明显的激活作用，Pb2＋、Mg2＋、Fe2＋、Mn2＋、Ca2＋、Fe3＋、Al3＋对酶有不同程度的抑制作用。
위연구흑곡매균주（Aspergillus niger ）XZ-3S 곡반발효목취당매조건급소산조매적성질，채용곡반（대소φ20 cm×4 cm）진행고태발효조건연구，병대소산조매진행매학성질측정。결과표명：재배양기간료100 g、초시료수비1∶1．7、초시 pH 자연상태、상대간물료접충량10％、중간정시번곡보수화28℃련속배양60 h 조건하，매활력최고가체24380 IU／g 간곡；조매최괄반응 pH5．0，최괄온도50℃；온도저우50℃、pH3．0～8．0적조건하매교은정；EDTA（활성물질）、Cu2＋、Co2＋대매유명현적격활작용，Pb2＋、Mg2＋、Fe2＋、Mn2＋、Ca2＋、Fe3＋、Al3＋대매유불동정도적억제작용。
The solid state fermentation condition of xylanase from A.niger XZ-3S strain and property of the produced crude enzyme were studied and determined by tray culture and enzyme activity method. The results showed that the xylanase activity can reach 24 380 IU/g under the fermentation conditions of 100g dry substance,1 ∶ 1.7 of dry substance :water,natural initial pH,10% inoculation size of dry substance,timing stirring and watering and 28℃ for 60h,and the optimal pH and temperature for the produced crude enzyme is 5.0 and 50℃ respectively.The property of the crude enzyme is stable under the conditions of below 50℃ and pH3.0~8.0.EDTA,Cu2+ and Co2+ have the obvious activation effect on activity of xylanase but Pb2+ ,Mg2+ ,Fe2+ ,Mn2+ ,Ca2+ ,Fe3+ and Al3+ have different inhibition effect on activity of xylanase to varying degrees respectively.