CAJ | 학술논문

为获得商洛五味子组培快繁的中间材料，选用幼嫩的商洛五味子茎尖、叶片、叶柄为外植体，探讨不同激素（2，4-D，NAA，6-BA）对3种外植体愈伤组织诱导的影响。结果表明：3种外植体愈伤组织诱导的最佳激素配比均为2，4-D 0．5 mg／L＋NAA 0．1 mg／L＋6-BA 1．0 mg／L。该配方处理下，茎尖诱导率达73．3％，愈伤组织呈绿色，质地致密，生长旺盛；叶柄诱导率达60．0％，愈伤组织呈淡绿色，质地较紧密，生长旺盛；叶愈伤组织诱导率相对较低（53．3％），诱导时间较长，3周后出现愈伤组织，呈绿色。商洛五味子茎尖为愈伤组织诱导的最佳外植体。
위획득상락오미자조배쾌번적중간재료，선용유눈적상락오미자경첨、협편、협병위외식체，탐토불동격소（2，4-D，NAA，6-BA）대3충외식체유상조직유도적영향。결과표명：3충외식체유상조직유도적최가격소배비균위2，4-D 0．5 mg／L＋NAA 0．1 mg／L＋6-BA 1．0 mg／L。해배방처리하，경첨유도솔체73．3％，유상조직정록색，질지치밀，생장왕성；협병유도솔체60．0％，유상조직정담록색，질지교긴밀，생장왕성；협유상조직유도솔상대교저（53．3％），유도시간교장，3주후출현유상조직，정록색。상락오미자경첨위유상조직유도적최가외식체。
In order to obtain intermediate materials for tissue culture of F.Schisandrae Chinensis in Shangluo ,with young fruit of F.Schisandrae Chinensis stem tip,leaf,petiole as explants,the author researches the effects of different hormones (2,4-D,NAA and 6-BA)on different explants callus induction of F.Schisandrae Chinensis in Shangluo.The results showed that the best medium of callus induction of F.Schisandrae Chinensis stem tip, leaf, petiole was 2,4-D 0.5 mg/L + 6-BA0.1 mg/L +NAA1.0 mg/L,stem tip induced rate of 73.3%,callus green,dense texture,thrive;Petiole induction rate was 60.0%,and the callus was pale green,quality of a material is close,thrive;Not callus induction rate is relatively low,the induction rate was 53.3%,the induction time is longer,three weeks after the callus,green.Stem tip of F.Schisandrae Chinensis in Shangluo is the best explant for callus induction.