CAJ | 학술논문

核酸检测技术广泛用于生命科学、微生物学及医学领域。随着分子生物学的发展,在经典技术的基础上研究人员又开发出多种新型检测技术。其中多重基因遗传表达分析系统(gene expression profiler genetic analysis system, GeXP)技术作为聚合酶链式反应和芯片的换代技术,具有特异性好、灵敏度高的特点,能够同时检测多个目的基因,真正意义上实现了高通量检测。本文详细介绍了 GeXP 技术的原理以及优缺点,并对GeXP技术在畜牧兽医研究、转基因食品检测、微生物检测及医学研究中的应用进行阐述,以期为该技术将来在食品检测方面的推广提供参考。
핵산검측기술엄범용우생명과학、미생물학급의학영역。수착분자생물학적발전,재경전기술적기출상연구인원우개발출다충신형검측기술。기중다중기인유전표체분석계통(gene expression profiler genetic analysis system, GeXP)기술작위취합매련식반응화심편적환대기술,구유특이성호、령민도고적특점,능구동시검측다개목적기인,진정의의상실현료고통량검측。본문상세개소료 GeXP 기술적원리이급우결점,병대GeXP기술재축목수의연구、전기인식품검측、미생물검측급의학연구중적응용진행천술,이기위해기술장래재식품검측방면적추엄제공삼고。
ABSTRACT:Detection of nucleic acid is widely used in life science, microbiology and medical science. With the developments of molecular biology, new testing approaches are constantly emerging on the basis of classical technology. As the regeneration technology of PCR and chip, gene expression profiler genetic analysis system (GeXP) has a broad application prospect, with features of high specificity and sensitivity, rapid speed and easy operation. This system truly realized the detection of high throughput for samples. This article reviewed the basic principle of GeXP system and its applications in veterinarian and animal husbandry, detection of genetically modified (GM) food and microorganism and medical research, which could provide a reference for detection of foodstuffs and microbiology.