CAJ | 학술논문

2013年初随着欧洲“马肉丑闻”这一食品安全事件的发生，食品掺假这一全球性问题引发了公众对食品安全的担忧以及各国政府的关注。用于食品真伪鉴别的分子生物学技术包括经典的普通PCR技术、实时定量PCR技术，以及近年来逐步发展起来的分子指纹技术等。此外，基因芯片技术、多重PCR技术等可提高检测通量的新型技术近年来也得到了长足发展。随着分子生物学技术向着高灵敏度、高通量的方向发展的同时，用于样品初筛的具有较高灵敏度和准确度的现场快速检测方法和检测设备的开发也将成为未来食品真伪鉴别的研究热点之一。本文对分子生物学技术在肉及肉制品真伪鉴别的应用和研究进展进行了综述。
2013년초수착구주“마육축문”저일식품안전사건적발생，식품참가저일전구성문제인발료공음대식품안전적담우이급각국정부적관주。용우식품진위감별적분자생물학기술포괄경전적보통PCR기술、실시정량PCR기술，이급근년래축보발전기래적분자지문기술등。차외，기인심편기술、다중PCR기술등가제고검측통량적신형기술근년래야득도료장족발전。수착분자생물학기술향착고령민도、고통량적방향발전적동시，용우양품초사적구유교고령민도화준학도적현장쾌속검측방법화검측설비적개발야장성위미래식품진위감별적연구열점지일。본문대분자생물학기술재육급육제품진위감별적응용화연구진전진행료종술。
ABSTRACT:With the food safety incident of “horse meat scandal” occurring in Europe in early 2013, the global problem of food adulteration has caused more concern about food safety from the public and the governments of many countries. Molecular techniques for food authenticity identification include the conventional PCR, real-time quantitative PCR, and fingerprinting techniques which developed in recent years. In addition, high throughput technologies such as gene chip technology and multiplex PCR technology have also developed considerably. At the same time, rapid on-site detection methods and equipment with high sensitivity and high-throughput for sample screening had become a research highlight in food authenticity identification. In this paper, molecular method research progress and the corresponding application on authenticity of meat and meat products were reviewed.