CAJ | 학술논문

目的：实现样品中牛源性成份和鸡源性成份的量化分析。方法通过在基因组单拷贝基因上设计引物，绘制模板DNA扩增标准曲线以及确定牛肉、鸡肉质量与DNA浓度的比值常数，利用实时荧光定量PCR技术对四种不同掺混比例的牛鸡瘦肉混合样本中牛源性成份和鸡源性成份所占的质量百分比含量进行分析。结果通过荧光实时定量PCR反应的Ct值、模板DNA扩增标准曲线和质量与DNA的比值常数可以计算出样品中所含牛源性成份和鸡源性成份的质量百分比含量，检测值与理论值之间的绝对误差可控制在5%以内，量化研究结果基本准确。结论对于组织成份单一的样品，可以通过在基因组单拷贝基因上设计特异性的引物，利用PCR 技术实现在质量水平上对食品中动物源性成份的量化分析，该技术方法的建立可以为肉类掺假监管工作提供有力的技术支撑。
목적：실현양품중우원성성빈화계원성성빈적양화분석。방법통과재기인조단고패기인상설계인물，회제모판DNA확증표준곡선이급학정우육、계육질량여DNA농도적비치상수，이용실시형광정량PCR기술대사충불동참혼비례적우계수육혼합양본중우원성성빈화계원성성빈소점적질량백분비함량진행분석。결과통과형광실시정량PCR반응적Ct치、모판DNA확증표준곡선화질량여DNA적비치상수가이계산출양품중소함우원성성빈화계원성성빈적질량백분비함량，검측치여이론치지간적절대오차가공제재5%이내，양화연구결과기본준학。결론대우조직성빈단일적양품，가이통과재기인조단고패기인상설계특이성적인물，이용PCR 기술실현재질량수평상대식품중동물원성성빈적양화분석，해기술방법적건립가이위육류참가감관공작제공유력적기술지탱。
ABSTRACT:Objective To quantitatively analyze the beef and chicken in blending samples.Methods The primers were designed according to the sequence of the single copy gene. A series of genome DNA were used as standard curve in real-time PCR. The mathematical conversion parameters about the mass of meat and DNA were determined. The weight/weight equivalents of beef and chicken in four blending samples were analyzed using real-time PCR.Results The weight/weight equivalents of beef and chicken was determined by calculated usingCt, standard curve and the mathematical conversion parameters about the mass of meat and DNA. The results showed that the absolute error of detection with a tolerance of less than 5%.Conclusion Quantitative analyzing of the composition of the animal species in single component sample could be realized using real-time PCR by designing the primer according to the sequence of genome. This research could provide technical support for food safety.