CAJ | 학술논문

目的：建立一种适用于中草药辐照检测方法。方法对白蔻、紫苏、忍冬藤、甘草与荷叶5种中草药样品分别在4 kGy、5 kGy及10 kGy剂量下进行辐照处理,同时以未辐照样品作为对照。辐照后分别测试每份样品的直接落射荧光计数单位(DEFT值)和细菌总数(APC值),并计算DEFT值与APC值之差(Dc值),将该差值与特定阈值比较判定样品辐照情况。结果随辐照剂量增加, DEFT值差异不显著, DEFT值分别为0 kGy：6.9~9.0,4 kGy：6.8~8.7,5 kGy：6.7~8.9,10 kGy：6.9~9.0； APC值逐渐降低, APC值分别为0 kGy：6.0~7.8,4 kGy：3.8~5.6,5 kGy：3.2~5.5,10 kGy：1.2~3.7；辐照后4组样品之间Dc值差异显著,4 kGy、5 kGy、10 kGy及0 kGy下辐照处理样品的Dc均值分别为2.6~4.9,3.0~5.3,5.3~7.3和0.4~2.0。结论当阈值设定为3.0时,可检测出5.0 kGy及5.0 kGy以上辐照处理样品。
목적：건립일충괄용우중초약복조검측방법。방법대백구、자소、인동등、감초여하협5충중초약양품분별재4 kGy、5 kGy급10 kGy제량하진행복조처리,동시이미복조양품작위대조。복조후분별측시매빈양품적직접락사형광계수단위(DEFT치)화세균총수(APC치),병계산DEFT치여APC치지차(Dc치),장해차치여특정역치비교판정양품복조정황。결과수복조제량증가, DEFT치차이불현저, DEFT치분별위0 kGy：6.9~9.0,4 kGy：6.8~8.7,5 kGy：6.7~8.9,10 kGy：6.9~9.0； APC치축점강저, APC치분별위0 kGy：6.0~7.8,4 kGy：3.8~5.6,5 kGy：3.2~5.5,10 kGy：1.2~3.7；복조후4조양품지간Dc치차이현저,4 kGy、5 kGy、10 kGy급0 kGy하복조처리양품적Dc균치분별위2.6~4.9,3.0~5.3,5.3~7.3화0.4~2.0。결론당역치설정위3.0시,가검측출5.0 kGy급5.0 kGy이상복조처리양품。
ABSTRACT:Objective To establish a screening method for detecting irradiation of herbal medicine in China.MethodsSamples of 5 kinds of herbal medicine, including cardamon, purple perilla, honeysuckle stem, liquorice, and lotus leaf, were irradiated with the doses of 4 kGy, 5 kGy and 10 kGy, one group of unirradiated samples were kept as the 0 kGy. The direct epifluorescent filter technique count unit (DEFT value), aerobic plate count unit (APC value), the difference of DEFT and APC (DC value) were calculated after irradiation. Then the DC value was compared to a special threshold to determine the irradiation situation of the samples.Results The results indicated that with the irradiation dose increased, the difference of DEFT was not significant, the DEFT value was 0 kGy: 6.9~9.0, 4 kGy: 6.8~8.7, 5 kGy: 6.7~8.9, 10 kGy: 6.9~9.0; and the APC value was decreased, the APC value was 0 kGy: 6.0~7.8, 4 kGy: 3.8~5.6, 5 kGy: 3.2~5.5, 10 kGy: 1.2~3.7; the Dc values of the 4 groups were statistically significant, the Dc values of 4 kGy, 5 kGy, 10 kGy and 0 kGy was 2.6~4.9, 3.0~5.3, 5.3~7.3 and 0.4~2.0 respectively.Conclusion When 3.0 was set as the threshold, the samples irradiated with dose of 5 kGy and up could be identified.