食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
2期
452-456
,共5页
庞璐%宋喆%吴冬雪%张海英%赵良娟%刘培%蔡国瑞%李宗梦%赵宏%张宏伟
龐璐%宋喆%吳鼕雪%張海英%趙良娟%劉培%蔡國瑞%李宗夢%趙宏%張宏偉
방로%송철%오동설%장해영%조량연%류배%채국서%리종몽%조굉%장굉위
单增李斯特氏菌%快速检测%PCR%免疫胶体金
單增李斯特氏菌%快速檢測%PCR%免疫膠體金
단증리사특씨균%쾌속검측%PCR%면역효체금
Listeria monocytogenes%rapid detection%PCR%immunogold method
目的：建立食品中单核增生李斯特氏菌快速检测PCR-免疫胶体金试纸条法。方法通过设计特异性引物建立单增李斯特氏菌检测PCR方法并使用免疫胶体金技术建立PCR产物快速检测试纸条；用试验菌株检测PCR-免疫胶体金试纸条方法的检测特异度与敏感度。使用新建方法对市售肉制品和乳制品中单核增生李斯特氏菌进行检测，验证该方法在食品检测中的可行性。结果 PCR-免疫胶体金法具有良好的特异度，敏感度比标准琼脂糖凝胶电泳法高100倍。采集乳品样品131份，阳性样品1份，检出率1.53%；肉制品224份，阳性样品4份，检测率1.79%。结论建立的单增李斯特氏菌检测PCR-免疫胶体金试纸条法特异度好，敏感度高，适用于食品中单增李斯特氏菌的检测。
目的：建立食品中單覈增生李斯特氏菌快速檢測PCR-免疫膠體金試紙條法。方法通過設計特異性引物建立單增李斯特氏菌檢測PCR方法併使用免疫膠體金技術建立PCR產物快速檢測試紙條；用試驗菌株檢測PCR-免疫膠體金試紙條方法的檢測特異度與敏感度。使用新建方法對市售肉製品和乳製品中單覈增生李斯特氏菌進行檢測，驗證該方法在食品檢測中的可行性。結果 PCR-免疫膠體金法具有良好的特異度，敏感度比標準瓊脂糖凝膠電泳法高100倍。採集乳品樣品131份，暘性樣品1份，檢齣率1.53%；肉製品224份，暘性樣品4份，檢測率1.79%。結論建立的單增李斯特氏菌檢測PCR-免疫膠體金試紙條法特異度好，敏感度高，適用于食品中單增李斯特氏菌的檢測。
목적：건립식품중단핵증생리사특씨균쾌속검측PCR-면역효체금시지조법。방법통과설계특이성인물건립단증리사특씨균검측PCR방법병사용면역효체금기술건립PCR산물쾌속검측시지조；용시험균주검측PCR-면역효체금시지조방법적검측특이도여민감도。사용신건방법대시수육제품화유제품중단핵증생리사특씨균진행검측，험증해방법재식품검측중적가행성。결과 PCR-면역효체금법구유량호적특이도，민감도비표준경지당응효전영법고100배。채집유품양품131빈，양성양품1빈，검출솔1.53%；육제품224빈，양성양품4빈，검측솔1.79%。결론건립적단증리사특씨균검측PCR-면역효체금시지조법특이도호，민감도고，괄용우식품중단증리사특씨균적검측。
ABSTRACT:Objective To establish the PCR-immunogold method for detection ofListeria monocytogenes in food.MethodsThe PCR method forListeria monocytogenes detection was built through the design of specific primers and the rapid test immunogold strip for detection of PCR products was established. Then the specificity and sensitivity of the new method were tested using the test bacterial strains andListeria monocytogenes in milk and meat products was detected using the PCR-immunogold strip method to verify whether this method is appropriate to food samples test.ResultsThe method established is specific and the sensitivity is higher than that of the agarose gel electrophoresis by 100 times. We screened 131 milk samples and 224 meat samples totally and found 1 (1.53%) of milk samples and 4 (1.79%) of meat samples carrying Listeria monocytogenes.ConclusionThe new method has high specificity and sensitivity and is suitable for the detection of Listeria monocytogenes in food.
