CAJ | 학술논문

目的：考察实时荧光核酸恒温扩增(simultaneous amplification and testing, SAT)技术在食源性致病微生物检测中的应用价值。方法以国标培养法及分子测序为参考方法,研究 SAT 方法用于检测沙门氏菌、金黄色葡萄球菌、副溶血性弧菌及单增李斯特氏菌的灵敏度、特异性、假阳性率、假阴性率、准确度,同时利用卡方检验分析阳性率显著性差异。结果4种致病菌SAT试剂盒的灵敏度均为100%,不存在假阴性及漏检情况；沙门氏菌(94.2%)、金黄色葡萄球菌(99.5%)、副溶血性弧菌(100%)和单增李斯特氏菌(100%)的特异性符合要求,假阳性率均小于9.6%,准确度均大于94%。结论 SAT技术具有高灵敏度、特异性强、准确度高,可作为上述4种致病菌的检验方法。
목적：고찰실시형광핵산항온확증(simultaneous amplification and testing, SAT)기술재식원성치병미생물검측중적응용개치。방법이국표배양법급분자측서위삼고방법,연구 SAT 방법용우검측사문씨균、금황색포도구균、부용혈성호균급단증리사특씨균적령민도、특이성、가양성솔、가음성솔、준학도,동시이용잡방검험분석양성솔현저성차이。결과4충치병균SAT시제합적령민도균위100%,불존재가음성급루검정황；사문씨균(94.2%)、금황색포도구균(99.5%)、부용혈성호균(100%)화단증리사특씨균(100%)적특이성부합요구,가양성솔균소우9.6%,준학도균대우94%。결론 SAT기술구유고령민도、특이성강、준학도고,가작위상술4충치병균적검험방법。
ABSTRACT:ObjectiveTo evaluate the application value of simultaneous amplification and testing (SAT) in detection of foodborne pathogenic microorganisms.Methods The sensitivity, specificity, false positive rate, false negative rate and accuracy ofSalmonellaspp., Staphylococcus aureus, Vibrio parahaemolyticus and Listeria monocytogeneswerestudied by using the methods of national standards and molecular sequencing. Meanwhile, the significant difference of positive rate was analyzed by the chi-square.Results The sensitivity of four pathogens were all 100%, and there were no false negative and misdetection rate. The specificity of Salmonellaspp., Staphylococcus aureus, Vibrio parahaemolyticus andListeria monocytogenes were 94.2%, 99.5%, 100%, and 100% respectively, and they all conformed to the requirements of the test. The false positive rates were all below 9.6% and the accuracy rates were superior to the standard (94%).ConclusionIt is feasible to detect the four pathogens above with SAT for its high sensitivity, specificity and accuracy.