CAJ | 학술논문

目的：建立辣椒油和火锅底料中的10种工业染料的检测方法。方法采用凝胶渗透色谱仪作为前处理设备，采用超高效液相色谱仪作为检测设备，对高脂肪含量的辣椒油及火锅底料等辣椒制品中的10种红黄类工业染料进行检测，并对方法的有效性进行验证。结果以空白的辣椒油和火锅底料为基质，分别在高、中、低三个水平进行加标实验，回收率分别在56.0%~96.4%之间和70.0%~98.2%之间， RSD (n=6)分别为1.9%~8.2%之间和1.8%~7.9%之间，各工业染料的检出限(S/N=3)在0.009~0.051 mg/kg之间，定量限(S/N=10)在0.030~0.153 mg/kg之间。结论该方法操作简单，稳定性好，适于在实际检测工作中推广应用。
목적：건립랄초유화화과저료중적10충공업염료적검측방법。방법채용응효삼투색보의작위전처리설비，채용초고효액상색보의작위검측설비，대고지방함량적랄초유급화과저료등랄초제품중적10충홍황류공업염료진행검측，병대방법적유효성진행험증。결과이공백적랄초유화화과저료위기질，분별재고、중、저삼개수평진행가표실험，회수솔분별재56.0%~96.4%지간화70.0%~98.2%지간， RSD (n=6)분별위1.9%~8.2%지간화1.8%~7.9%지간，각공업염료적검출한(S/N=3)재0.009~0.051 mg/kg지간，정량한(S/N=10)재0.030~0.153 mg/kg지간。결론해방법조작간단，은정성호，괄우재실제검측공작중추엄응용。
ABSTRACT:ObjectiveTo establish a method for the determination of 10 dyes in hot pot material and chili oil.MethodsTen dyes in chili oil, hotpot material and other foods with abundant fat were detected by gel permeation chromatography (GPC) with ultra performance liquid chromatography (UPLC). At the same time the effectiveness was verified.ResultsSpiking experiments were carried out in high, medium and low level, respectively. Recoveries of chili oil and hotpot material were between 56.0%~96.4% and 70.0% to 98%, the RSD (n=6) were between 1.9% to 8.2% and 1.8% to 7.9%, the limits of detection (S/N=3) were between 0.009~0.051 mg/kg, and the limits of quantification (S/N=10) were between 0.030~0.153 mg/kg.Conclusion This method is simple to operate with good stability and is suitable for the application in daily testing work.