食品安全质量检测学报
食品安全質量檢測學報
식품안전질량검측학보
FOOD SAFETY AND QUALITY DETECTION TECHNOLOGY
2015年
2期
602-608
,共7页
贾子才%徐杰%宋雨%李兆杰%薛长湖
賈子纔%徐傑%宋雨%李兆傑%薛長湖
가자재%서걸%송우%리조걸%설장호
高效液相色谱法%蒸发光散射检测法%神经酰胺%脑苷脂%海参
高效液相色譜法%蒸髮光散射檢測法%神經酰胺%腦苷脂%海參
고효액상색보법%증발광산사검측법%신경선알%뇌감지%해삼
high performance liquid chromatography%evaporative light-scattering detection%ceramides%cerebrosides%sea cucumber
目的：建立了一种高效液相色谱-蒸发光散射检测方法(high performance liquid chromatography coupled with evaporative light-scattering detection, HPLC-ELSD)同时分析海参中脑苷脂和神经酰胺含量的方法。方法以TSKgel CN-80Ts柱为分离柱,正己烷-异丙醇-二氯甲烷-甲醇为流动相,采用梯度洗脱方式,流速1.0 mL/min,漂移管温度50℃,氮气流速2.4 L/min。结果在优化条件下,18 min内完成分离,脑苷脂的线性范围为2.5~250μg/mL,检出限为0.5μg/mL(S/N=3),相关系数R>0.99,神经酰胺的线性范围为5~150μg/mL,检出限为0.05μg/mL(S/N=3),相关系数R>0.99,方法稳定性良好；不同种海参中脑苷脂和神经酰胺含量存在较为明显的差异,含量范围分别为0~4.44 mg/g和0.24~2.21 mg/g。其中,猪参的脑苷脂含量最高为4.44 mg/g,而白底靴参的脑苷脂未能检出。阿拉斯加红参的神经酰胺含量最高为2.21 mg/g,而海地瓜神经酰胺含量最少。结论所建立的方法灵敏、准确、快速,可应用于食品中脑苷脂和神经酰胺的同时定量分析。
目的：建立瞭一種高效液相色譜-蒸髮光散射檢測方法(high performance liquid chromatography coupled with evaporative light-scattering detection, HPLC-ELSD)同時分析海參中腦苷脂和神經酰胺含量的方法。方法以TSKgel CN-80Ts柱為分離柱,正己烷-異丙醇-二氯甲烷-甲醇為流動相,採用梯度洗脫方式,流速1.0 mL/min,漂移管溫度50℃,氮氣流速2.4 L/min。結果在優化條件下,18 min內完成分離,腦苷脂的線性範圍為2.5~250μg/mL,檢齣限為0.5μg/mL(S/N=3),相關繫數R>0.99,神經酰胺的線性範圍為5~150μg/mL,檢齣限為0.05μg/mL(S/N=3),相關繫數R>0.99,方法穩定性良好；不同種海參中腦苷脂和神經酰胺含量存在較為明顯的差異,含量範圍分彆為0~4.44 mg/g和0.24~2.21 mg/g。其中,豬參的腦苷脂含量最高為4.44 mg/g,而白底靴參的腦苷脂未能檢齣。阿拉斯加紅參的神經酰胺含量最高為2.21 mg/g,而海地瓜神經酰胺含量最少。結論所建立的方法靈敏、準確、快速,可應用于食品中腦苷脂和神經酰胺的同時定量分析。
목적：건립료일충고효액상색보-증발광산사검측방법(high performance liquid chromatography coupled with evaporative light-scattering detection, HPLC-ELSD)동시분석해삼중뇌감지화신경선알함량적방법。방법이TSKgel CN-80Ts주위분리주,정기완-이병순-이록갑완-갑순위류동상,채용제도세탈방식,류속1.0 mL/min,표이관온도50℃,담기류속2.4 L/min。결과재우화조건하,18 min내완성분리,뇌감지적선성범위위2.5~250μg/mL,검출한위0.5μg/mL(S/N=3),상관계수R>0.99,신경선알적선성범위위5~150μg/mL,검출한위0.05μg/mL(S/N=3),상관계수R>0.99,방법은정성량호；불동충해삼중뇌감지화신경선알함량존재교위명현적차이,함량범위분별위0~4.44 mg/g화0.24~2.21 mg/g。기중,저삼적뇌감지함량최고위4.44 mg/g,이백저화삼적뇌감지미능검출。아랍사가홍삼적신경선알함량최고위2.21 mg/g,이해지과신경선알함량최소。결론소건립적방법령민、준학、쾌속,가응용우식품중뇌감지화신경선알적동시정량분석。
ABSTRACT:ObjectiveTo establisha high performance liquid chromatography coupled with evaporative light-scattering detection (HPLC-ELSD) method for analyzing the contents of cerebrosides and ceramides simultaneously in sea cucumbers.Methods The separation was performed on a TSK gel CN-80Ts column by usingn-hexane-isopropanol-dichloromethane-methanol as the mobile phase at a flow rate of 1.0 mL/min. The temperature of drift tube was kept at 50℃ and the flow rate of nitrogen was 2.4 L/min. Seventeen sea cucumber species were selected as experimental subjects. ResultsUnder optimal conditions, effective separation of cerebrosides and ceramides was completed within 18 min. The linear range of the established cerebrosides calibration curve was 2.5~250 μg/mL, and the limit of detection was 0.5 μg/mL with a regression correlation coefficient of 0.99. Meanwhile, the linear range of the established ceramides calibration curve was 5~150 μg/mL, and the limit of detection was 0.05μg/mL with a regression correlation coefficient of 0.99. There were obvious difference in cerebrosides and ceramides among these sea cucumber species. The cerebrosides content ofBohadschia marmoratawas the highest, which was up to 4.44 mg/g, in contrast, that of Actinopyga mauritiana could not be detected. As for ceramides, the content ofParastichopus californicus was the highest, which was up to 2.21 mg/g, in contrast,Acaudina molpadioides had the lowest content. Conclusion The established method can provide essential data for development and utilization of sea cucumbers, which is sensitive, accurate and fast.
