CAJ | 학술논문

目的：建立可同时检测掺入羊肉中的猪、马、牛、鸭成分的多重荧光PCR检测体系。方法配制掺入猪、马、牛、鸭源性成分的模拟羊肉掺假样品，掺假比例17%~45%。筛选特异性强、敏感度高的猪、马、牛、鸭物种特异性基因，优化反应体系，建立了针对猪、牛、马、鸭两两配对的两重荧光PCR检测体系和其中3个任意组合的三重荧光PCR检测体系。结果模拟羊肉掺假样品两重荧光PCR检测结果显示，包括单个成分掺入比例低至7%的样品，其检测准确率为100%。而三重荧光PCR检测同时检测猪、牛、马、鸭四种肉品中的3种，其中猪肉、牛肉和马肉的掺假比例均为8%~15%；鸭牛肉掺假比例为5%~10%。三重荧光PCR检测结果显示，所有掺假样品中的各种掺假组分都被准确检出。结论本研究建立的多重荧光 PCR 检测体系能准确检出配制的模拟掺假羊肉制品中的猪、马、牛、鸭成分，可进一步研究以应用于市场肉制品及加工肉制品掺假的检测。
목적：건립가동시검측참입양육중적저、마、우、압성분적다중형광PCR검측체계。방법배제참입저、마、우、압원성성분적모의양육참가양품，참가비례17%~45%。사선특이성강、민감도고적저、마、우、압물충특이성기인，우화반응체계，건립료침대저、우、마、압량량배대적량중형광PCR검측체계화기중3개임의조합적삼중형광PCR검측체계。결과모의양육참가양품량중형광PCR검측결과현시，포괄단개성분참입비례저지7%적양품，기검측준학솔위100%。이삼중형광PCR검측동시검측저、우、마、압사충육품중적3충，기중저육、우육화마육적참가비례균위8%~15%；압우육참가비례위5%~10%。삼중형광PCR검측결과현시，소유참가양품중적각충참가조분도피준학검출。결론본연구건립적다중형광 PCR 검측체계능준학검출배제적모의참가양육제품중적저、마、우、압성분，가진일보연구이응용우시장육제품급가공육제품참가적검측。
ABSTRACT:Objective To establish multiplex fluorescent polymerase chain reaction (PCR) system, and simultaneously detect pig, horse, cattle and duck meat mixed in mutton. Methods Mutton samples were mixed with 17%~45% pig, horse, cattle and duck meat. The specific species genes were selected with good specificity and sensitivity to form random duplex and triplex fluorescent PCR detection system for pig, cattle, horse and duck. ResultsThe duplex fluorescent PCR system could identify single added component as low as 7% for 100% accuracy. The triplex fluorescent PCR system could identify 8%~15% pig,cattle, horse and 5%~10% duck in any combination for 100% accuracy.Conclusion The established multiplex fluorescent PCR system can detect pig, horse, cattle and duck meat mixed in prepared mutton samples accurately. It can be further researched to application in market meat and processed meat adulteration detection.