CAJ | 학술논문

目的：探讨胃癌细胞herg mRNA、HERG蛋白表达及HERG电流强度的变化的临床意义。方法培养胃癌细胞（胃癌细胞系SGC7901、MGC803、AGS、MKN45）及永生化胃上皮细胞（ GES ），取对数生长期细胞用于实验。采用RT-PCR法检测herg mRNA表达，Western blot法检测HERG蛋白表达，采用全细胞膜片钳技术测定SGC7901及GES的HERG电流强度。结果 herg mRNA及其蛋白在4种胃癌细胞系中均有表达，AGS中HERG蛋白表达量低于其他三种细胞系（ P均＜0．05）；GES中无herg mRNA及其蛋白表达。在SGC7901中检测到HERG电流， GES中未记录到HERG电流。结论 herg mRNA及其蛋白在胃癌细胞系SGC7901、MGC803、AGS、MKN45表达增高， SGC7901中存在HERG电流。 HERG蛋白可能参与了胃癌的发生，并与胃癌恶性程度有关。
목적：탐토위암세포herg mRNA、HERG단백표체급HERG전류강도적변화적림상의의。방법배양위암세포（위암세포계SGC7901、MGC803、AGS、MKN45）급영생화위상피세포（ GES ），취대수생장기세포용우실험。채용RT-PCR법검측herg mRNA표체，Western blot법검측HERG단백표체，채용전세포막편겸기술측정SGC7901급GES적HERG전류강도。결과 herg mRNA급기단백재4충위암세포계중균유표체，AGS중HERG단백표체량저우기타삼충세포계（ P균＜0．05）；GES중무herg mRNA급기단백표체。재SGC7901중검측도HERG전류， GES중미기록도HERG전류。결론 herg mRNA급기단백재위암세포계SGC7901、MGC803、AGS、MKN45표체증고， SGC7901중존재HERG전류。 HERG단백가능삼여료위암적발생，병여위암악성정도유관。
Objective To investigate the expression of herg gene , HERG protein and HERG current in gastric cancer cells.Methods Gastric cancer cells and gastric epithelial ( GES) cells were cultured to logarithmic phase .The expres-sions of herg mRNA and HERG protein in gastric cancer cells and GES cells were measured by using RT -PCR and Western blot, respectively.The whole cell configuration of the patch-clamp technique was employed to record HERG currents in va-rious cells .Results HERG mRNA and protein were positively expressed in four gastric cancer cell lines .Expression level of HERG protein in AGS cells was lower compared with the other three gastric cancer cell lines (P<0.05).There was negative expression of herg mRNA and HERG protein in GES cell line .HERG current was detected in gastric cancer cell , whereas there was no HERG current in GES cell .Conclusion Herg gene and HERG protein were highly expressed in gas-tric cancer cells and HERG current was exclusively detected in gastric cancer cells .HERG protein was associated with car-cinogenesis of gastric cancer and may serve as a diagnostic marker for gastric cancer .