CAJ | 학술논문

目的：阐明非酒精性脂肪性肝病(NAFLD)患者肠道菌群结构特征。方法采用454焦磷酸测序技术，对47例经“肝活检”确诊的NAFLD患者和34例健康人新鲜粪便样品16S DNA V3～V5可变区进行测序，通过序列比较及操作分类单元（OTU）划分评价肠道菌群结构差异。结果 NAFLD患者OTUs为（88.32±28.27），显著低于健康人[（109.65±30.65），P<0.01]；在“门”水平，NAFLD患者厚壁菌门所占比例为（51.2±17.8）%，显著高于健康人的[（46.4±12.8）%，P=0.048]，而拟杆菌门为（31.6±18.9）%，显著低于健康人的[（43.3±14.4）%，P<0.001]；在“纲”水平上，NAFLD组Erysipelotrichi纲占（3.2±5.1）%，显著高于健康人的[（1.0±1.2）%，P=0.009]，而Bacteroidia纲占（31.0±18.8）%，显著低于对照组的[（42.3±14.0）%，P=0.004]；在“属”水平上，NAFLD组乳球菌占（0.0038±0.0001）%，低于健康组的[（0.0145±0.001）%，P=0.003]，普氏菌属也同样显著减少（P=0.022），而链球菌（Streptococcus）在NAFLD占（1.50±0.03）%，显著高于对照组的[（0.21±0.24）%，P=0.004]。结论 NAFLD患者肠道菌群多样性减少，存在构成显著异常，是致病因素抑或其后果仍有待于进一步研究。
목적：천명비주정성지방성간병(NAFLD)환자장도균군결구특정。방법채용454초린산측서기술，대47례경“간활검”학진적NAFLD환자화34례건강인신선분편양품16S DNA V3～V5가변구진행측서，통과서렬비교급조작분류단원（OTU）화분평개장도균군결구차이。결과 NAFLD환자OTUs위（88.32±28.27），현저저우건강인[（109.65±30.65），P<0.01]；재“문”수평，NAFLD환자후벽균문소점비례위（51.2±17.8）%，현저고우건강인적[（46.4±12.8）%，P=0.048]，이의간균문위（31.6±18.9）%，현저저우건강인적[（43.3±14.4）%，P<0.001]；재“강”수평상，NAFLD조Erysipelotrichi강점（3.2±5.1）%，현저고우건강인적[（1.0±1.2）%，P=0.009]，이Bacteroidia강점（31.0±18.8）%，현저저우대조조적[（42.3±14.0）%，P=0.004]；재“속”수평상，NAFLD조유구균점（0.0038±0.0001）%，저우건강조적[（0.0145±0.001）%，P=0.003]，보씨균속야동양현저감소（P=0.022），이련구균（Streptococcus）재NAFLD점（1.50±0.03）%，현저고우대조조적[（0.21±0.24）%，P=0.004]。결론 NAFLD환자장도균군다양성감소，존재구성현저이상，시치병인소억혹기후과잉유대우진일보연구。
Objective To elucidate the changes of gut microbiota projects in patients with non-alcoholic fat-ty liver diseases(NAFLD). Methods Fresh faecal samples from 47 NAFLD patients underwent liver biopsies and 34 health individuals were examined using bar coded 454 pyrosequencing technology. 16S DNA V3~V5 variable regions were sequenced and the operational taxonomy unit (OUT) was analyzed to evaluate the differences of mi-crobiota projects. Results The OUT of patients with NAFLD were (88.32±28.27),significantly lower than those in the controls [(109.65±30.65),P<0.01)];In Phylum level,the abundance of Firmicutes had significantly increased in NAFLD patients than in the controls [(51.2 ±17.8)% vs.(46.4 ±12.8)%,P=0.048],while the abundance of Bac-teroidetes had significantly reduced [(31.0 ±18.8)% vs.(43.3 ±14.4)%,P<0.01];In Class level,the abundance of Erysipelotrichi had significantly increased in NAFLD patients than in the controls [(3.2±5.1)% vs. (1.0±1.2)%,P=0.009],while the abundance of Bacteroidia had significantly decreased [(31.0±18.8)% vs.(42.3±14.0)%,P=0.004];In Genus level,the Lactococcus [(0.0038±0.0001)%] had significantly reduced in NAFLD patients compared with controls [(0.0145±0.001)%,P=0.003];The same results were found in Prevotella Genus. However,Streptococcus had significantly increased in NAFLD patients [(1.50 ±0.03)%] than in controls (0.21 ±0.24)%,P=0.004]. Conclu-sions Patients with NAFLD had a lower diversity of gut microbiota projects.