应用化工
應用化工
응용화공
APPLIED CHEMICAL INDUSTRY
2015年
2期
369-370,385
,共3页
青荚叶%豆甾醇%含量测定%反相高效液相色谱法
青莢葉%豆甾醇%含量測定%反相高效液相色譜法
청협협%두치순%함량측정%반상고효액상색보법
Helwingia%stigmasterol%content determination%HPLC
采用反相高效液相色谱法( RP-HPLC )测定青荚叶中豆甾醇的含量,色谱柱为 Thermo C18柱(250 mm ×4.6 mm,5μm),流动相为甲醇:水(97:3),流速1.0 mL/min,进样量20μL,检测波长210 nm。结果表明,豆甾醇进样量在0.216~2.16μg,其峰面积与浓度呈良好的线性关系( R2=0.9995),回收率为97.79%。方法专属性强,准确可靠,可用于青荚叶中豆甾醇的含量测定。
採用反相高效液相色譜法( RP-HPLC )測定青莢葉中豆甾醇的含量,色譜柱為 Thermo C18柱(250 mm ×4.6 mm,5μm),流動相為甲醇:水(97:3),流速1.0 mL/min,進樣量20μL,檢測波長210 nm。結果錶明,豆甾醇進樣量在0.216~2.16μg,其峰麵積與濃度呈良好的線性關繫( R2=0.9995),迴收率為97.79%。方法專屬性彊,準確可靠,可用于青莢葉中豆甾醇的含量測定。
채용반상고효액상색보법( RP-HPLC )측정청협협중두치순적함량,색보주위 Thermo C18주(250 mm ×4.6 mm,5μm),류동상위갑순:수(97:3),류속1.0 mL/min,진양량20μL,검측파장210 nm。결과표명,두치순진양량재0.216~2.16μg,기봉면적여농도정량호적선성관계( R2=0.9995),회수솔위97.79%。방법전속성강,준학가고,가용우청협협중두치순적함량측정。
Using reversed-phase high performance liquid chromatography( RP-HPLC )to determine the content of stigmasterol in Helwingia,the chromatographic column was Thermo C18 column( 250 mm × 4. 6 mm,5μm),the mobile phase of methanol:water(97:3),flow rate of 1. 0 mL/min,the injection vol-ume was 20 μL,the detection wavelength was 210 nm. Results showed that stigmasterol sample volume in 0. 216~2. 16μg range,there was a good linear relationship between the peak area and concentration(R2=0. 999 5),the recovery rate was 97. 79%. This method is specific,accurate and reliable,and can be used for content determination of stigmasterol in Helwingia.