CAJ | 학술논문

目的：本研究旨在探讨扶正化瘀组分复方的抗肝纤维化作用及其作用机制。方法：采用DMN诱导小鼠肝纤维化模型，以扶正化瘀方为阳性对照。以天狼猩红染色和羟脯氨酸含量评估肝纤维化程度；肝脏微血管成像和CD31标记微血管密度评价肝脏血管新生；western blot法检测肝组织VEGF-R2表达；通过计数转基因斑马鱼功能性节间血管数和碱性磷酸酶活性验证药物对血管的影响。结果：扶正化瘀组分复方可显著改善纤维化小鼠血清肝功能（P＜0.01）：减少肝组织胶原沉积（P＜0.01）；减少肝脏微血管数量（P＜0.01）；下调VEGFR2蛋白表达（P＜0.01）；抑制斑马鱼碱性磷酸酶活性。结论：扶正化瘀组分复方具有抑制DMN诱导小鼠肝纤维化模型肝组织纤维化的作用，其作用机制可能与抑制血管新生有关。
목적：본연구지재탐토부정화어조분복방적항간섬유화작용급기작용궤제。방법：채용DMN유도소서간섬유화모형，이부정화어방위양성대조。이천랑성홍염색화간포안산함량평고간섬유화정도；간장미혈관성상화CD31표기미혈관밀도평개간장혈관신생；western blot법검측간조직VEGF-R2표체；통과계수전기인반마어공능성절간혈관수화감성린산매활성험증약물대혈관적영향。결과：부정화어조분복방가현저개선섬유화소서혈청간공능（P＜0.01）：감소간조직효원침적（P＜0.01）；감소간장미혈관수량（P＜0.01）；하조VEGFR2단백표체（P＜0.01）；억제반마어감성린산매활성。결론：부정화어조분복방구유억제DMN유도소서간섬유화모형간조직섬유화적작용，기작용궤제가능여억제혈관신생유관。
Objective:The purpose of this study is to investigate the mechanism of Fuzheng Huayu Components formula against Liver Fibrosis relating to Angiogenesis.Methods:Using DMN-induced liver fibrosis model in mice,Fuzheng Huayu formula as positive control.Liver fibrosis was estimated by Sirius red staining and hydroxyproline content assay.Liver angiogenesis was evalu-ated by liver tissue microvascular imaging analysis and MVD labeled by CD31 .VEGF-R2 expression was observed by western blot. Zebrafish functional intersegmental vessels count and alkaline phosphatase activity was to investigate angiogenesis.Results:Fuzheng Huayu Components formula can alleviate liver function in DMN mice (P <0.01 ).Liver collagen deposition and hydroxyproline content were reduced significantly (P<0.01 ).The hepatic microvasculature reduced(P<0.01 )with the VEG-FR2 protein expression down regulated accordingly(P<0.01 ).It also inhibited alkaline phosphatase activity in zebrafish.Con-clusion:Fuzheng Huayu Components formula inhibited liver fibrosis in DMN-treated mice.Its mechanism may be related to inhib-it angiogenesis.