世界中医药
世界中醫藥
세계중의약
WORLD CHINESE MEDICINE
2015年
2期
178-181
,共4页
肝糖原%急性肝损伤%PAS染色法%改良
肝糖原%急性肝損傷%PAS染色法%改良
간당원%급성간손상%PAS염색법%개량
Hepatic glycogen%Acute hepatic injury%Periodic acid-schiff (PAS)%Improvement
目的：观察改良肝脏糖原PAS染色法，并观察肝脏糖原染色在急性肝损伤中的应用。方法：复制CCl4急性肝损伤模型，首次100％CCl43 mL／kg皮下注射，此后50％CCl4橄榄油溶液2 mL／kg每周2次共4次皮下注射，诱导大鼠急性肝损伤模型。计算大鼠肝体比；HE染色观察肝组织炎症病理；试剂盒检测血清丙氨酸氨基转移酶（ALT）、天门冬氨酸氨基转移酶（AST）、总胆红素（TBil）、白蛋白（Alb）。肝脏常规PAS染色与改良PAS染色观察肝糖原染色。结果：与正常组相比，模型组ALT、AST活性与TBil含量明显升高（P＜0.05），Alb含量明显降低（P＜0.05）；HE染色示，模型组肝小叶结构排列紊乱，肝细胞脂肪变、气球样变明显。常规PAS染色，正常组肝组织PAS染色阳性占肝脏面积为32.38％±5.50％；与正常组相比，模型组肝组织PAS阳性染色明显减少（P＜0.01），占肝脏面积为8.60％±3.34％。改良PAS染色提示，正常组肝脏可见大量PAS阳性染色，占肝脏面积为75.50％±9.02％；与正常组相比，模型组肝组织PAS阳性染色明显减少（P＜0.01），占肝脏面积为17.61％±3.53％。在空白对照组与模型肝组织中，肝糖原改良PAS染色阳性率明显高于常规PAS染色法（P＜0.01）。改良PAS染色肝糖原阳性染色面积更真实反映急性肝损伤程度。结论：改良肝脏糖原PAS染色法有助于急性肝损伤程度评估。
目的：觀察改良肝髒糖原PAS染色法，併觀察肝髒糖原染色在急性肝損傷中的應用。方法：複製CCl4急性肝損傷模型，首次100％CCl43 mL／kg皮下註射，此後50％CCl4橄欖油溶液2 mL／kg每週2次共4次皮下註射，誘導大鼠急性肝損傷模型。計算大鼠肝體比；HE染色觀察肝組織炎癥病理；試劑盒檢測血清丙氨痠氨基轉移酶（ALT）、天門鼕氨痠氨基轉移酶（AST）、總膽紅素（TBil）、白蛋白（Alb）。肝髒常規PAS染色與改良PAS染色觀察肝糖原染色。結果：與正常組相比，模型組ALT、AST活性與TBil含量明顯升高（P＜0.05），Alb含量明顯降低（P＜0.05）；HE染色示，模型組肝小葉結構排列紊亂，肝細胞脂肪變、氣毬樣變明顯。常規PAS染色，正常組肝組織PAS染色暘性佔肝髒麵積為32.38％±5.50％；與正常組相比，模型組肝組織PAS暘性染色明顯減少（P＜0.01），佔肝髒麵積為8.60％±3.34％。改良PAS染色提示，正常組肝髒可見大量PAS暘性染色，佔肝髒麵積為75.50％±9.02％；與正常組相比，模型組肝組織PAS暘性染色明顯減少（P＜0.01），佔肝髒麵積為17.61％±3.53％。在空白對照組與模型肝組織中，肝糖原改良PAS染色暘性率明顯高于常規PAS染色法（P＜0.01）。改良PAS染色肝糖原暘性染色麵積更真實反映急性肝損傷程度。結論：改良肝髒糖原PAS染色法有助于急性肝損傷程度評估。
목적：관찰개량간장당원PAS염색법，병관찰간장당원염색재급성간손상중적응용。방법：복제CCl4급성간손상모형，수차100％CCl43 mL／kg피하주사，차후50％CCl4감람유용액2 mL／kg매주2차공4차피하주사，유도대서급성간손상모형。계산대서간체비；HE염색관찰간조직염증병리；시제합검측혈청병안산안기전이매（ALT）、천문동안산안기전이매（AST）、총담홍소（TBil）、백단백（Alb）。간장상규PAS염색여개량PAS염색관찰간당원염색。결과：여정상조상비，모형조ALT、AST활성여TBil함량명현승고（P＜0.05），Alb함량명현강저（P＜0.05）；HE염색시，모형조간소협결구배렬문란，간세포지방변、기구양변명현。상규PAS염색，정상조간조직PAS염색양성점간장면적위32.38％±5.50％；여정상조상비，모형조간조직PAS양성염색명현감소（P＜0.01），점간장면적위8.60％±3.34％。개량PAS염색제시，정상조간장가견대량PAS양성염색，점간장면적위75.50％±9.02％；여정상조상비，모형조간조직PAS양성염색명현감소（P＜0.01），점간장면적위17.61％±3.53％。재공백대조조여모형간조직중，간당원개량PAS염색양성솔명현고우상규PAS염색법（P＜0.01）。개량PAS염색간당원양성염색면적경진실반영급성간손상정도。결론：개량간장당원PAS염색법유조우급성간손상정도평고。
Objective:To study in situ staining of glycogen by modified Periodic acid-schiff (PAS)staining on evaluating acute hepatic injury induced by carbon tetrachloride (CCl4)in rats.Methods:Rats were randomly divided into 2 groups:normal group and model group.Except normal rats,all the other rats in model group were injected subcutaneously 1 00% CCl4 3mL/kg once, then 50% CCl4 2mL/kg two times a week for 4 times.All the rats were sacrificed on the 1 5th day.Liver body ratio of rats was calculated;The serum parameters of alanine aminotransferase (ALT),aspartate aminotransferase (AST),total bilirubin (TBil), albumin (Alb)were assayed.Hepatic inflammation was observed with HE staining.Hepatic glycogen was stained by routine PAS staining and improved PAS staining.Results:Compared with the normal group,the activities of ALT and AST and TBil content in-creased significantly (P<0.05 ).Alb content was significantly decreased compared with the normal group (P<0.05 ).HE stai-ning showed that serious structural damage,hepatic steatosis and ballooning degeneration were seen in model group.Routine PAS staining showed that PAS positive staining area accounted for 32.38% ±5.50% in normal group.Compared with the normal group,PAS positive staining area decreased significantly (8.60%±3.34 %,P<0.01 ).Improved PAS staining showed that PAS positive staining area accounted for 75 .50%±9.02 % in normal group.Compared with the normal group,Improved PAS positive staining area decreased significantly (1 7.61%±3.53%,P<0.01 ).In normal group and model group,improved hepat-ic glycogen staining showed positive staining of PAS was significantly higher than that of routine hepatic glycogen staining (P<0.01 ).This suggests that the improved PAS staining of liver glycogen positive stained area more truly reflected the degree of acute liver injury.Conclusion:The improved of Periodic acid-schiff (PAS)staining method is helpful to improve the evaluation quality degree of acute hepatic injury.
