山东医药
山東醫藥
산동의약
SHANDONG MEDICAL JOURNAL
2015年
7期
1-4
,共4页
脊髓损伤%支架材料%神经营养素3%维甲酸%神经干细胞
脊髓損傷%支架材料%神經營養素3%維甲痠%神經榦細胞
척수손상%지가재료%신경영양소3%유갑산%신경간세포
spinal cord injuries%scaffold materials%neurotrophin-3%retinoic acid%neural stem cells
目的：构建明胶—壳聚糖复合神经营养素3神经支架材料，并检测其生物相容性。方法混合明胶、壳聚糖溶液后加入微量神经营养素3，将混浊液注模成型后冷淋干燥制备明胶—壳聚糖复合神经营养素3神经支架材料，利用扫描电子显微镜观察支架材料形态，液体代替法测算孔隙率。提取明胶—壳聚糖复合神经营养素3神经支架材料浸提液，观察其对神经干细胞活性的影响及对全反式维甲酸预诱导的神经干细胞分化的影响，并采用膜片钳技术检测诱导分化前后细胞的电生理特性。结果明胶—壳聚糖复合神经营养素3神经支架材料内径为（267．0±13．8）μm，孔隙率为90．0％。神经干细胞在支架材料上生长良好，在全反式维甲酸诱导下形态向神经元样细胞改变，并初步表现出神经元间突触连接的结构，且诱导分化的神经元样细胞初步具备神经元细胞的电生理特性。结论成功构建了明胶—壳聚糖复合神经营养素3神经支架材料，其与神经干细胞之间的生物相容性良好。
目的：構建明膠—殼聚糖複閤神經營養素3神經支架材料，併檢測其生物相容性。方法混閤明膠、殼聚糖溶液後加入微量神經營養素3，將混濁液註模成型後冷淋榦燥製備明膠—殼聚糖複閤神經營養素3神經支架材料，利用掃描電子顯微鏡觀察支架材料形態，液體代替法測算孔隙率。提取明膠—殼聚糖複閤神經營養素3神經支架材料浸提液，觀察其對神經榦細胞活性的影響及對全反式維甲痠預誘導的神經榦細胞分化的影響，併採用膜片鉗技術檢測誘導分化前後細胞的電生理特性。結果明膠—殼聚糖複閤神經營養素3神經支架材料內徑為（267．0±13．8）μm，孔隙率為90．0％。神經榦細胞在支架材料上生長良好，在全反式維甲痠誘導下形態嚮神經元樣細胞改變，併初步錶現齣神經元間突觸連接的結構，且誘導分化的神經元樣細胞初步具備神經元細胞的電生理特性。結論成功構建瞭明膠—殼聚糖複閤神經營養素3神經支架材料，其與神經榦細胞之間的生物相容性良好。
목적：구건명효—각취당복합신경영양소3신경지가재료，병검측기생물상용성。방법혼합명효、각취당용액후가입미량신경영양소3，장혼탁액주모성형후랭림간조제비명효—각취당복합신경영양소3신경지가재료，이용소묘전자현미경관찰지가재료형태，액체대체법측산공극솔。제취명효—각취당복합신경영양소3신경지가재료침제액，관찰기대신경간세포활성적영향급대전반식유갑산예유도적신경간세포분화적영향，병채용막편겸기술검측유도분화전후세포적전생리특성。결과명효—각취당복합신경영양소3신경지가재료내경위（267．0±13．8）μm，공극솔위90．0％。신경간세포재지가재료상생장량호，재전반식유갑산유도하형태향신경원양세포개변，병초보표현출신경원간돌촉련접적결구，차유도분화적신경원양세포초보구비신경원세포적전생리특성。결론성공구건료명효—각취당복합신경영양소3신경지가재료，기여신경간세포지간적생물상용성량호。
Objective To fabricate the gelatin-chitosan composite neurotrophins-3 nerve scaffolds and to determine its biocompatibility .Methods We mixed the gelatin and chitosan solutions , then added a trace of neurotrophin-3.After mak-ing the injection mold , we fabricated the nerve guidance scaffolds by the freeze-drying technique .Then the characteristics of the scaffold were observed by scanning electron microscope morphology , and the scaffold diameter , porosity, etc were de-tected.We extracted the gelatin chitosan composite neurotrophin-3 nerve scaffold material extracts , and then we observed the effects on the growth and differentiation of neural stem cells;the electrophysiological properties of the differentiated cells were observed by the patch-clamp technique .Results The inside diameter of gelatin-chitosan composite neurotrophins-3 nerve scaffold was (267.0 ±13.8) μm and porosity was 90.0%.Scanning electron microscopy showed that neural stem cells grew well on the scaffold.Under the induction of retinoic acid , neural stem cells differentiated into neuron-like cells, and showed structural of synaptic connections between neurons initially .What′s more, the cells differentiated were detected the neuron-like cell electrophysiological properties by the patch-clamp technique .Conclusion The gelatin-chitosan com-posite neurotrophin-3 nerve scaffolds were successfully constructed , and had good biocompatibility with neural stem cells .
