中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
2期
192-195
,共4页
组织构建%软骨组织工程%双醋瑞因%骨关节炎%软骨细胞%结缔组织生长因子%白细胞介素1
組織構建%軟骨組織工程%雙醋瑞因%骨關節炎%軟骨細胞%結締組織生長因子%白細胞介素1
조직구건%연골조직공정%쌍작서인%골관절염%연골세포%결체조직생장인자%백세포개소1
Osteoarthritis%Chondrocytes%Connective Tissue Growth Factor%Interleukin-1
背景:在骨关节炎软骨退变中结缔组织生长因子作为一种重要的效应分子在软骨细胞的增殖、分化方面发挥重要作用。临床应用双醋瑞因治疗骨关节炎已取得了良好的效果,但其治疗的确切机制尚不清楚。目的:观察不同浓度双醋瑞因对体外白细胞介素1β诱导下软骨细胞中结缔组织生长因子的影响。方法:体外培养SD大鼠关节软骨细胞,重组人白细胞介素1β刺激软骨细胞制备体外骨关节炎模型。实验分组:正常对照组不给予任何处理因素;模型对照组给予重组人白细胞介素1β;实验组给予不同浓度双醋瑞因+10μg/L重组人白细胞介素1β。利用MTT比色法观察软骨细胞的增殖情况,Western Blot法检测结缔组织生长因子的表达。以上实验均重复3次。结果与结论:MTT结果显示,与正常对照组比较,双醋瑞因能促进软骨细胞MTT增殖活性,以浓度为10-5 mol/L更明显(P <0.01),白细胞介素1β作用后各实验组软骨细胞增殖能力下降(P <0.05);但与正常对照组比较,无论是否加白细胞介素1β,浓度为10-4 mol/L和10-5 mol/L双醋瑞因仍能促进软骨细胞MTT增殖活性(P <0.05)。Western Blot检测结果显示,白细胞介素1β能够降低结缔组织生长因子的表达(P <0.01),浓度为10-5 mol/L双醋瑞因能够显著促进白细胞介素1β诱导下结缔组织生长因子的表达,显著高于模型对照组(P<0.01)。提示双醋瑞因可以促进白细胞介素1β诱导下结缔组织生长因子的高表达,其可能是双醋瑞因促进软骨细胞的分化增殖,治疗骨关节炎的作用机制之一。
揹景:在骨關節炎軟骨退變中結締組織生長因子作為一種重要的效應分子在軟骨細胞的增殖、分化方麵髮揮重要作用。臨床應用雙醋瑞因治療骨關節炎已取得瞭良好的效果,但其治療的確切機製尚不清楚。目的:觀察不同濃度雙醋瑞因對體外白細胞介素1β誘導下軟骨細胞中結締組織生長因子的影響。方法:體外培養SD大鼠關節軟骨細胞,重組人白細胞介素1β刺激軟骨細胞製備體外骨關節炎模型。實驗分組:正常對照組不給予任何處理因素;模型對照組給予重組人白細胞介素1β;實驗組給予不同濃度雙醋瑞因+10μg/L重組人白細胞介素1β。利用MTT比色法觀察軟骨細胞的增殖情況,Western Blot法檢測結締組織生長因子的錶達。以上實驗均重複3次。結果與結論:MTT結果顯示,與正常對照組比較,雙醋瑞因能促進軟骨細胞MTT增殖活性,以濃度為10-5 mol/L更明顯(P <0.01),白細胞介素1β作用後各實驗組軟骨細胞增殖能力下降(P <0.05);但與正常對照組比較,無論是否加白細胞介素1β,濃度為10-4 mol/L和10-5 mol/L雙醋瑞因仍能促進軟骨細胞MTT增殖活性(P <0.05)。Western Blot檢測結果顯示,白細胞介素1β能夠降低結締組織生長因子的錶達(P <0.01),濃度為10-5 mol/L雙醋瑞因能夠顯著促進白細胞介素1β誘導下結締組織生長因子的錶達,顯著高于模型對照組(P<0.01)。提示雙醋瑞因可以促進白細胞介素1β誘導下結締組織生長因子的高錶達,其可能是雙醋瑞因促進軟骨細胞的分化增殖,治療骨關節炎的作用機製之一。
배경:재골관절염연골퇴변중결체조직생장인자작위일충중요적효응분자재연골세포적증식、분화방면발휘중요작용。림상응용쌍작서인치료골관절염이취득료량호적효과,단기치료적학절궤제상불청초。목적:관찰불동농도쌍작서인대체외백세포개소1β유도하연골세포중결체조직생장인자적영향。방법:체외배양SD대서관절연골세포,중조인백세포개소1β자격연골세포제비체외골관절염모형。실험분조:정상대조조불급여임하처리인소;모형대조조급여중조인백세포개소1β;실험조급여불동농도쌍작서인+10μg/L중조인백세포개소1β。이용MTT비색법관찰연골세포적증식정황,Western Blot법검측결체조직생장인자적표체。이상실험균중복3차。결과여결론:MTT결과현시,여정상대조조비교,쌍작서인능촉진연골세포MTT증식활성,이농도위10-5 mol/L경명현(P <0.01),백세포개소1β작용후각실험조연골세포증식능력하강(P <0.05);단여정상대조조비교,무론시부가백세포개소1β,농도위10-4 mol/L화10-5 mol/L쌍작서인잉능촉진연골세포MTT증식활성(P <0.05)。Western Blot검측결과현시,백세포개소1β능구강저결체조직생장인자적표체(P <0.01),농도위10-5 mol/L쌍작서인능구현저촉진백세포개소1β유도하결체조직생장인자적표체,현저고우모형대조조(P<0.01)。제시쌍작서인가이촉진백세포개소1β유도하결체조직생장인자적고표체,기가능시쌍작서인촉진연골세포적분화증식,치료골관절염적작용궤제지일。
BACKGROUND:In osteoarthritis cartilage degeneration, connective tissue growth factors as the important effector molecules play an important role in chondrocyte proliferation and differentiation. Clinical application of diacerein for the treatment of osteoarthritis has achieved good effect, but the exact mechanism of the treatment is unclear. OBJECTIVE:To observe the effect of different concentrations of diacerein on the expression of connective tissue growth factor in the articular cartilage induced with interleukin-1βin vitro. METHODS: Chondrocytes of Sprague Dawley rats were culturedin vitro, and then the chondrocytes were induced with recombinant human interleukin-1β to prepare the osteoarthritis model. Rats in the normal control group received no treatment; rats in the model group were treated with recombinant human interleukin-1β; rats in the experimental group were treated with different concentrations of diacerein+10 μg/L recombinant human interleukin-1β. The proliferation of chondrocytes was observed withMTT assay, and Western blot was used to detect the expression of connective tissue growth factor. The experiments above were repeated for three times. RESULTS AND CONCLUSION:The MTT assay results showed that compared with the normal control group, the diacerein could promote the proliferation activity of chondrocytes, and more significant in the concentration of 10-5 mol/L (P < 0.01); the proliferation capacity of chondrocytes in the experimental group was decreased after induced with interleukin-1β (P < 0.05), but when compared with the normal control group, the diacerein with the concentrations of 10-4 mol/L and 10-5 mol/L could promote the MTT proliferation capacity of chondrocytes with or without interleukin-1β (P < 0.05). Western blot results showed that interleukin-1β could reduce the expression of connective tissue growth factor (P< 0.01), and 10-5 mol/L diacerein could significantly promote the expression of connective tissue growth factor induced with interleukin-1β, and the effect was significantly higher than that in the model group (P < 0.01). Diacerein can promote expression of connective tissue growth factor induced with interleukin-1β, it may be one the mechanism of diacerein for promoting chondrocytes differentiation and proliferation and the treatment of osteoarthritis.