中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
2期
177-181
,共5页
组织构建%骨组织工程%骨关节炎%chemerin%酶联免疫吸附试验
組織構建%骨組織工程%骨關節炎%chemerin%酶聯免疫吸附試驗
조직구건%골조직공정%골관절염%chemerin%매련면역흡부시험
Knee Osteoarthritis%Cytokines%Enzyme-Linked Immunosorbent Assay
背景：研究发现，chemerin受刺激后可进一步扩增炎症信号，导致炎症因子和基质金属蛋白酶的释放。提示chemerin可能作为软骨代谢的重要调控因子，在骨关节炎的病理过程中发挥重要的作用。目的：分析膝骨关节炎患者关节液和血清中chemerin水平与骨关节炎严重程度的相关性。方法：选择经临床确诊的膝骨关节炎患者80例，采用酶联免疫吸附试验(ELISA)法对关节液和血清中的chemerin水平进行检测，骨关节炎影像学严重程度评分采用国际公认的Kel gren-Lawrence(K-L)评分系统。结果与结论：患者关节液和血清中chemerin水平与Kelgren-Lawrence分级有显著相关性(r=0.981，P<0.001和r=0.901，P <0.001)，且血清中chemerin水平与关节液中chemerin水平呈正相关(r=0.596，P<0.01)。结果表明，骨关节炎患者关节液和血清中chemerin水平与影像学K-L分级严重程度正相关，chemerin水平可作为骨关节炎诊断及判断严重程度的参考指标。
揹景：研究髮現，chemerin受刺激後可進一步擴增炎癥信號，導緻炎癥因子和基質金屬蛋白酶的釋放。提示chemerin可能作為軟骨代謝的重要調控因子，在骨關節炎的病理過程中髮揮重要的作用。目的：分析膝骨關節炎患者關節液和血清中chemerin水平與骨關節炎嚴重程度的相關性。方法：選擇經臨床確診的膝骨關節炎患者80例，採用酶聯免疫吸附試驗(ELISA)法對關節液和血清中的chemerin水平進行檢測，骨關節炎影像學嚴重程度評分採用國際公認的Kel gren-Lawrence(K-L)評分繫統。結果與結論：患者關節液和血清中chemerin水平與Kelgren-Lawrence分級有顯著相關性(r=0.981，P<0.001和r=0.901，P <0.001)，且血清中chemerin水平與關節液中chemerin水平呈正相關(r=0.596，P<0.01)。結果錶明，骨關節炎患者關節液和血清中chemerin水平與影像學K-L分級嚴重程度正相關，chemerin水平可作為骨關節炎診斷及判斷嚴重程度的參攷指標。
배경：연구발현，chemerin수자격후가진일보확증염증신호，도치염증인자화기질금속단백매적석방。제시chemerin가능작위연골대사적중요조공인자，재골관절염적병리과정중발휘중요적작용。목적：분석슬골관절염환자관절액화혈청중chemerin수평여골관절염엄중정도적상관성。방법：선택경림상학진적슬골관절염환자80례，채용매련면역흡부시험(ELISA)법대관절액화혈청중적chemerin수평진행검측，골관절염영상학엄중정도평분채용국제공인적Kel gren-Lawrence(K-L)평분계통。결과여결론：환자관절액화혈청중chemerin수평여Kelgren-Lawrence분급유현저상관성(r=0.981，P<0.001화r=0.901，P <0.001)，차혈청중chemerin수평여관절액중chemerin수평정정상관(r=0.596，P<0.01)。결과표명，골관절염환자관절액화혈청중chemerin수평여영상학K-L분급엄중정도정상관，chemerin수평가작위골관절염진단급판단엄중정도적삼고지표。
BACKGROUND:Studies have found that after stimulation, chemerin can further amplify the inflammatory signal, resulting in the release of inflammatory factors and matrix metaloproteinases. It indicates that chemerin may serve as an important regulator of cartilage metabolism, and plays an important role in the pathogenesis of osteoarthritis. OBJECTIVE: To explore the correlation between level of chemerin in synovia and serum and severity of knee osteoarthritis. METHODS: Eighty patients with knee osteoarthritis were enroled in the study. Knee osteoarthritis grading was evaluated according to the Kelgren-Lawrence classification. Chemerin levels in synovia and serum were examined with enzyme-linked immunosorbent assay. RESULTS AND CONCLUSION:The level of chemerin in synovia and serum in patients with knee osteoarthritis were positively correlated with the Kelgren-Lawrence classification (r=0.981,P< 0.001;r=0.901,P < 0.001). And there was a positive correlation between the level of chemerin in synovia and in serum (r=0.596,P < 0.01). Experimental findings suggest that the levels of chemerin in synovial fluid and serum that are positively correlated with the Kelgren-Lawrence classification can be used as a reference biomarker of severity of knee osteoarthritis.
