中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
2期
170-176
,共7页
沈耿杨%任辉%江晓兵%梁德%杨志东%唐晶晶%崔健超%林顺鑫%庄洪%张顺聪%姚珍松
瀋耿楊%任輝%江曉兵%樑德%楊誌東%唐晶晶%崔健超%林順鑫%莊洪%張順聰%姚珍鬆
침경양%임휘%강효병%량덕%양지동%당정정%최건초%림순흠%장홍%장순총%요진송
组织构建%骨组织工程%去卵巢大鼠%不同时期%骨量%Ⅰ型前胶原氨基端原肽%Ⅰ型胶原羧基端肽%雌激素%变化规律%相关性%绝经后骨质疏松症%广东省自然科学基金
組織構建%骨組織工程%去卵巢大鼠%不同時期%骨量%Ⅰ型前膠原氨基耑原肽%Ⅰ型膠原羧基耑肽%雌激素%變化規律%相關性%絕經後骨質疏鬆癥%廣東省自然科學基金
조직구건%골조직공정%거란소대서%불동시기%골량%Ⅰ형전효원안기단원태%Ⅰ형효원최기단태%자격소%변화규률%상관성%절경후골질소송증%광동성자연과학기금
Osteoporosis,Postmenopausal%Estrogens%Ovariectomy
背景：目前对去卵巢大鼠的研究较多，而对不同时间点大鼠骨量、骨转换指标、雌激素水平的变化规律及各因素的相关性研究报道较少。目的：分析去卵巢大鼠不同时期骨量、骨转换指标、雌激素水平的变化规律并探讨其相关性。方法：34只3月龄雌性SD大鼠，随机分为基线组、假手术组和去卵巢组。实验开始先将基线组处死，假手术组及去卵巢组于术后第4，8，12周分次处死。双能X射线吸收法(DXA)测定L 1-3结果与结论：①去卵巢后4周去卵巢组离体腰椎及股骨骨矿含量、骨密度均较基线组、假手术组明显降低(P <0.05)，第8，12周时均显著改善(P <0.05)，腰椎、股骨各区域骨量丢失幅度最大的为L及股骨不同分区(头颈部R1区、转子部R2区、股骨干R3区、股骨整体R4区)的骨矿含量、骨密度、骨面积；酶联免疫吸附法(ELISA)检测血清Ⅰ型前胶原氨基端原肽、Ⅰ型胶原羧基端肽及雌激素水平。对大鼠体质量、离体骨密度、Ⅰ型前胶原氨基端原肽、Ⅰ型胶原羧基端肽、雌激素水平、月龄间的相关性进行分析。1及股骨转子区。②去卵巢后4周去卵巢组血清Ⅰ型前胶原氨基端原肽、Ⅰ型胶原羧基端肽水平较基线组、假手术组均显著升高(P <0.05)，第8，12周差异无显著性意义。③去卵巢组第8，12周血清雌激素较假手术组及基线组明显降低(P<0.01，P <0.05)。④月龄与大鼠体质量、腰椎及股骨骨密度呈正相关，Ⅰ型前胶原氨基端原肽、Ⅰ型胶原羧基端肽与腰椎及股骨骨密度呈负相关(P<0.01)。提示去卵巢后大鼠腰椎、股骨骨量变化呈先快速降低、再缓慢回升的趋势，其中L1及股骨转子部受影响最大；骨转换指标在去卵巢后显著加快、后期逐渐回归正常；雌激素水平变化规律为第1个月先升高、后期快速降低；体质量、骨转换指标及雌激素水平与骨量密切相关。
揹景：目前對去卵巢大鼠的研究較多，而對不同時間點大鼠骨量、骨轉換指標、雌激素水平的變化規律及各因素的相關性研究報道較少。目的：分析去卵巢大鼠不同時期骨量、骨轉換指標、雌激素水平的變化規律併探討其相關性。方法：34隻3月齡雌性SD大鼠，隨機分為基線組、假手術組和去卵巢組。實驗開始先將基線組處死，假手術組及去卵巢組于術後第4，8，12週分次處死。雙能X射線吸收法(DXA)測定L 1-3結果與結論：①去卵巢後4週去卵巢組離體腰椎及股骨骨礦含量、骨密度均較基線組、假手術組明顯降低(P <0.05)，第8，12週時均顯著改善(P <0.05)，腰椎、股骨各區域骨量丟失幅度最大的為L及股骨不同分區(頭頸部R1區、轉子部R2區、股骨榦R3區、股骨整體R4區)的骨礦含量、骨密度、骨麵積；酶聯免疫吸附法(ELISA)檢測血清Ⅰ型前膠原氨基耑原肽、Ⅰ型膠原羧基耑肽及雌激素水平。對大鼠體質量、離體骨密度、Ⅰ型前膠原氨基耑原肽、Ⅰ型膠原羧基耑肽、雌激素水平、月齡間的相關性進行分析。1及股骨轉子區。②去卵巢後4週去卵巢組血清Ⅰ型前膠原氨基耑原肽、Ⅰ型膠原羧基耑肽水平較基線組、假手術組均顯著升高(P <0.05)，第8，12週差異無顯著性意義。③去卵巢組第8，12週血清雌激素較假手術組及基線組明顯降低(P<0.01，P <0.05)。④月齡與大鼠體質量、腰椎及股骨骨密度呈正相關，Ⅰ型前膠原氨基耑原肽、Ⅰ型膠原羧基耑肽與腰椎及股骨骨密度呈負相關(P<0.01)。提示去卵巢後大鼠腰椎、股骨骨量變化呈先快速降低、再緩慢迴升的趨勢，其中L1及股骨轉子部受影響最大；骨轉換指標在去卵巢後顯著加快、後期逐漸迴歸正常；雌激素水平變化規律為第1箇月先升高、後期快速降低；體質量、骨轉換指標及雌激素水平與骨量密切相關。
배경：목전대거란소대서적연구교다，이대불동시간점대서골량、골전환지표、자격소수평적변화규률급각인소적상관성연구보도교소。목적：분석거란소대서불동시기골량、골전환지표、자격소수평적변화규률병탐토기상관성。방법：34지3월령자성SD대서，수궤분위기선조、가수술조화거란소조。실험개시선장기선조처사，가수술조급거란소조우술후제4，8，12주분차처사。쌍능X사선흡수법(DXA)측정L 1-3결과여결론：①거란소후4주거란소조리체요추급고골골광함량、골밀도균교기선조、가수술조명현강저(P <0.05)，제8，12주시균현저개선(P <0.05)，요추、고골각구역골량주실폭도최대적위L급고골불동분구(두경부R1구、전자부R2구、고골간R3구、고골정체R4구)적골광함량、골밀도、골면적；매련면역흡부법(ELISA)검측혈청Ⅰ형전효원안기단원태、Ⅰ형효원최기단태급자격소수평。대대서체질량、리체골밀도、Ⅰ형전효원안기단원태、Ⅰ형효원최기단태、자격소수평、월령간적상관성진행분석。1급고골전자구。②거란소후4주거란소조혈청Ⅰ형전효원안기단원태、Ⅰ형효원최기단태수평교기선조、가수술조균현저승고(P <0.05)，제8，12주차이무현저성의의。③거란소조제8，12주혈청자격소교가수술조급기선조명현강저(P<0.01，P <0.05)。④월령여대서체질량、요추급고골골밀도정정상관，Ⅰ형전효원안기단원태、Ⅰ형효원최기단태여요추급고골골밀도정부상관(P<0.01)。제시거란소후대서요추、고골골량변화정선쾌속강저、재완만회승적추세，기중L1급고골전자부수영향최대；골전환지표재거란소후현저가쾌、후기축점회귀정상；자격소수평변화규률위제1개월선승고、후기쾌속강저；체질량、골전환지표급자격소수평여골량밀절상관。
BACKGROUND:There are so many studies about ovariectomized rats at present, but the research on the change rules of bone mass, bone turnover markers, estrogen levels and their correlation in different periods of ovariectomized rats is rarely reported. OBJECTIVE:To analyze the change rules of bone mass, bone turnover markers, estrogen level and their correlation in different periods of ovariectomized rats. METHODS: Thirty-four 3-month-old female Sprague Dawley rats were randomly divided into three groups: baseline group, ovariectomized group and sham operated group. At the beginning of the experiment, the rats in the baseline group were sacrificed, then rats in the ovariectomized group and sham operated group were executed at 4, 8, 12 weeks postoperative respectively. The bone mineral density, bone mass content, area of different zones of the L1-3 lumbar vertebrae and femurs were detected by dual-energy X-ray absorption method, and meanwhile the serum levels of type I procolagen amino-terminal pro-peptide, I colagen carboxy-terminal peptide and estrogen were determined by ELISA. At last, we analyzed the correlation between body mass, bone mineral densityin vitro, type I procolagen amino-terminal pro-peptide, I colagen carboxy-terminal peptide and estrogen levels and the age of ovariectomized rats. RESULTS AND CONCLUSION: (1) The bone mineral density and bone mass content of the lumbar vertebral and femurs in the ovariectomized group were significantly lower than those in the sham operated group and baseline group at the 4th week after operation (P < 0.05). The bone mineral density and bone mass content in the ovariectomized group were ameliorated obviously at the 8th and 12th weeks compared with those at the 4th week after operation (P < 0.05). The bone mass loss was highest in the L1 and intertrochanteric regions. (2) Serum levels of type I procolagen amino-terminal pro-peptide and I colagen carboxy-terminal peptide in the ovariectomized group were significantly higher than those in the baseline group and sham operated group at the 4th week after operation, but there was no difference at the 8th and 12th weeks. (3) The serum estrogen level in the ovariectomized group was prominently lower than that in the sham operated group and baseline group at the 8th and 12th weeks after operation (P < 0.01 at the 8th week,P < 0.05 at the 12th week). (4) The age was positively correlated with body mass and bone mineral density of the lumbar vertebrae and femursin vitro, while the serum levels of type I procolagen amino-terminal pro-peptide and I colagen carboxy-terminal peptide were negatively correlated with the bone mineral density of the lumbar vertebrae and femurs in vitro (P < 0.01). These results suggested that the bone mass of the lumbar vertebrae and femurs in ovariectomized rats was decreased rapidly firstly, and then rose slowly with time; the bone mass in the L1 and intertrochanteric regions lost seriously; the bone turnover markers showed a significant increase at the beginning of ovariectomy and reduced gradualy to normal condition, while the estrogen level was increased at the first month after ovariectomy and then decreased rapidly. In addition, the body mass, bone turnover markers and estrogen level were associated with the change of bone mass.
