中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
5期
783-787
,共5页
郝志强%张涛%李孟彬%王为忠%张洪伟
郝誌彊%張濤%李孟彬%王為忠%張洪偉
학지강%장도%리맹빈%왕위충%장홍위
实验动物%移植%丙酮酸%移植小肠%缺血再灌注损伤%保护作用%细胞间黏附分子%模型,动物%大鼠
實驗動物%移植%丙酮痠%移植小腸%缺血再灌註損傷%保護作用%細胞間黏附分子%模型,動物%大鼠
실험동물%이식%병동산%이식소장%결혈재관주손상%보호작용%세포간점부분자%모형,동물%대서
Pyruvate%Reperfusion Injury%Intercel ular Adhesion Molecular 1%Smal Intestine%Transplantation%Models,Animal
背景：小肠移植过程中移植肠不可避免地要受到缺血再灌注的损害，而小肠又对缺血再灌注损伤特别敏感。前期研究证实丙酮酸对小肠和移植小肠缺血再灌注损伤具有保护作用，并探讨了部分相关机制。
<br> 目的：在前期研究基础上，进一步证实探讨丙酮酸对大鼠移植小肠缺血再灌注损伤的保护作用与移植小肠组织中细胞间黏附分子表达的关系。
<br> 方法：选用近交系成年雄性SD大鼠，按随机数字表法分为3组：假手术组行剖腹、左侧肾切除作为对照；移植小肠缺血再灌注组和丙酮酸处理组均建立小肠移植缺血再灌注动物模型，分别于供体小肠阻断血流、灌洗前10 min向肠腔内注入10 mL含有多聚葡萄糖的营养液或含有分析纯丙酮酸的营养液。分别留取缺血45，90 min和再灌注30，180 min小肠组织标本，光镜下观察小肠组织损伤病理变化，免疫组化法检测小肠组织中细胞间黏附分子1的表达。
<br> 结果与结论：缺血再灌注不同时相移植小肠缺血再灌注组小肠组织损伤程度均重于其他2组，而丙酮酸处理组小肠组织损伤程度与假手术组相似。缺血期间小肠组织中细胞间黏附分子1的表达均不明显，呈弱阳性；再灌注后移植小肠缺血再灌注组细胞间黏附分子1的表达迅速增加，高于与假手术组及丙酮酸处理组(P <0.01)，而丙酮酸处理组细胞间黏附分子1的表达变化不明显(P>0.05)。说明丙酮酸作用下大鼠移植小肠缺血再灌注过程中细胞间黏附分子1的表达减少，可能是丙酮酸保护大鼠移植小肠缺血再灌注损伤的重要环节之一。
揹景：小腸移植過程中移植腸不可避免地要受到缺血再灌註的損害，而小腸又對缺血再灌註損傷特彆敏感。前期研究證實丙酮痠對小腸和移植小腸缺血再灌註損傷具有保護作用，併探討瞭部分相關機製。
<br> 目的：在前期研究基礎上，進一步證實探討丙酮痠對大鼠移植小腸缺血再灌註損傷的保護作用與移植小腸組織中細胞間黏附分子錶達的關繫。
<br> 方法：選用近交繫成年雄性SD大鼠，按隨機數字錶法分為3組：假手術組行剖腹、左側腎切除作為對照；移植小腸缺血再灌註組和丙酮痠處理組均建立小腸移植缺血再灌註動物模型，分彆于供體小腸阻斷血流、灌洗前10 min嚮腸腔內註入10 mL含有多聚葡萄糖的營養液或含有分析純丙酮痠的營養液。分彆留取缺血45，90 min和再灌註30，180 min小腸組織標本，光鏡下觀察小腸組織損傷病理變化，免疫組化法檢測小腸組織中細胞間黏附分子1的錶達。
<br> 結果與結論：缺血再灌註不同時相移植小腸缺血再灌註組小腸組織損傷程度均重于其他2組，而丙酮痠處理組小腸組織損傷程度與假手術組相似。缺血期間小腸組織中細胞間黏附分子1的錶達均不明顯，呈弱暘性；再灌註後移植小腸缺血再灌註組細胞間黏附分子1的錶達迅速增加，高于與假手術組及丙酮痠處理組(P <0.01)，而丙酮痠處理組細胞間黏附分子1的錶達變化不明顯(P>0.05)。說明丙酮痠作用下大鼠移植小腸缺血再灌註過程中細胞間黏附分子1的錶達減少，可能是丙酮痠保護大鼠移植小腸缺血再灌註損傷的重要環節之一。
배경：소장이식과정중이식장불가피면지요수도결혈재관주적손해，이소장우대결혈재관주손상특별민감。전기연구증실병동산대소장화이식소장결혈재관주손상구유보호작용，병탐토료부분상관궤제。
<br> 목적：재전기연구기출상，진일보증실탐토병동산대대서이식소장결혈재관주손상적보호작용여이식소장조직중세포간점부분자표체적관계。
<br> 방법：선용근교계성년웅성SD대서，안수궤수자표법분위3조：가수술조행부복、좌측신절제작위대조；이식소장결혈재관주조화병동산처리조균건립소장이식결혈재관주동물모형，분별우공체소장조단혈류、관세전10 min향장강내주입10 mL함유다취포도당적영양액혹함유분석순병동산적영양액。분별류취결혈45，90 min화재관주30，180 min소장조직표본，광경하관찰소장조직손상병리변화，면역조화법검측소장조직중세포간점부분자1적표체。
<br> 결과여결론：결혈재관주불동시상이식소장결혈재관주조소장조직손상정도균중우기타2조，이병동산처리조소장조직손상정도여가수술조상사。결혈기간소장조직중세포간점부분자1적표체균불명현，정약양성；재관주후이식소장결혈재관주조세포간점부분자1적표체신속증가，고우여가수술조급병동산처리조(P <0.01)，이병동산처리조세포간점부분자1적표체변화불명현(P>0.05)。설명병동산작용하대서이식소장결혈재관주과정중세포간점부분자1적표체감소，가능시병동산보호대서이식소장결혈재관주손상적중요배절지일。
BACKGROUND:Grafted smal bowels are sensitive to ischemia-reperfusion injury, which is inevitable during transplantation process. Our previous study found the protective effect of pyruvate to rat grafted smal bowel and investigated the underlying mechanisms.
<br> OBJECTIVE:To study the relationship between pyruvate protective effect on ischemia-reperfusion injury and expression of intercel ular adhesion molecular 1 of grafted smal bowel.
<br> METHODS:Adult male Sprague-Dawley rats of inbred line were randomly divided into three groups. Sham operation group:Rats underwent the laparotomy and the left nephrectomy. Ischemia/reperfusion group and pyruvate group:Ischemia/reperfusion rat models of smal bowel transplantation were established and received lavage injection of nutrient solution only or nutrient solution with pyruvate, before donor intestinal blocking blood flow. Smal intestinal tissue was harvested at 45, 90 minutes after ischemia and 30, 180 minutes after reperfusion. The pathological changes of smal intestinal injury were observed under light microscope. The expression of intercel ular adhesion molecular 1 was detected by immunohistochemistry in the smal intestine tissue samples. RESULTS AND CONCLUSION:The pathological injury of smal intestine tissue at different time points of ischemia/reperfusion was severer in the ischemia/reperfusion group than that in sham operation group and pyruvate group, and there was no significant difference in the latter two groups. The expression of intercel ular adhesion molecular 1 stayed at a low level during ischemia period, and appeared sharp rise during reperfusion period, which was higher than that in the sham operation group and pyruvate group (P<0.01). No significant differences were found between sham operation group and pyruvate group in the expression (P>0.05). We conclude that pyruvate can afford protective effect to ischemia/reperfusion injury of grafted smal bowel. The mechanism of protective effect of pyruvate is associated with decrease expression of intercel ular adhesion molecular 1.
