中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
5期
772-777
,共6页
唐伟伟%习小庆%胡红林%黄雅为%叶真逢%陈定义
唐偉偉%習小慶%鬍紅林%黃雅為%葉真逢%陳定義
당위위%습소경%호홍림%황아위%협진봉%진정의
实验动物%泌尿系统损伤模型%肾缺血再灌注损伤%微小核糖核酸%BALB/C小鼠%性别差异%基因芯片%聚类分析%苏木精-伊红染色%江西省自然科学基金
實驗動物%泌尿繫統損傷模型%腎缺血再灌註損傷%微小覈糖覈痠%BALB/C小鼠%性彆差異%基因芯片%聚類分析%囌木精-伊紅染色%江西省自然科學基金
실험동물%비뇨계통손상모형%신결혈재관주손상%미소핵당핵산%BALB/C소서%성별차이%기인심편%취류분석%소목정-이홍염색%강서성자연과학기금
Tissue Engineering%Kidney%Reperfusion Injury%Microchip Analytical Procedures
背景：有研究表明肾缺血再灌注损伤存在性别差异，但其具体机制有待进一步研究。<br> 目的：观察雌雄性小鼠缺血前后肾脏组织中MicroRNA表达及其差异，以期进一步研究MicroRNA在肾缺血再灌注损伤性别差异中的作用及机制。<br> 方法：将雄性和雌性小鼠分别肾缺血45 min再灌注损伤24 h，同时设置雄性和雌性假手术组作对照。采用MicroRNA基因芯片技术检测雌雄小鼠肾缺血45 min再灌注损伤24 h及假手术后肾组织MicroRNA表达的差异，样品间表达差异的阈值为2倍。<br> 结果与结论：在雌性肾缺血再灌注与雄性肾缺血再灌注组对比发现有表达上调的MicroRNA有5个；雌性肾缺血再灌注组与雌性假手术组对比发现有差异表达的MicroRNA有29个，其中上调的有MicroRNA有25个，下调的MicroRNA有4个；雄性肾缺血再灌注组与雄性假手术组对比发现有差异表达的MicroRNA有38个，其中上调的MicroRNA有9个，下调的MicroRNA有29个；雌性假手术组与雄性假手术组对比发现有差异表达的MicroRNA有102个，其中上调MicroRNA的有22个，下调的MicroRNA有80个。结果说明，不同性别小鼠肾缺血再灌注前后肾组织中微小核糖核酸表达存在差异，这些 MicroRNA 的差异表达可能导致不同性别小鼠肾脏对缺血再灌注损伤的敏感性及耐受性存在差异。
揹景：有研究錶明腎缺血再灌註損傷存在性彆差異，但其具體機製有待進一步研究。<br> 目的：觀察雌雄性小鼠缺血前後腎髒組織中MicroRNA錶達及其差異，以期進一步研究MicroRNA在腎缺血再灌註損傷性彆差異中的作用及機製。<br> 方法：將雄性和雌性小鼠分彆腎缺血45 min再灌註損傷24 h，同時設置雄性和雌性假手術組作對照。採用MicroRNA基因芯片技術檢測雌雄小鼠腎缺血45 min再灌註損傷24 h及假手術後腎組織MicroRNA錶達的差異，樣品間錶達差異的閾值為2倍。<br> 結果與結論：在雌性腎缺血再灌註與雄性腎缺血再灌註組對比髮現有錶達上調的MicroRNA有5箇；雌性腎缺血再灌註組與雌性假手術組對比髮現有差異錶達的MicroRNA有29箇，其中上調的有MicroRNA有25箇，下調的MicroRNA有4箇；雄性腎缺血再灌註組與雄性假手術組對比髮現有差異錶達的MicroRNA有38箇，其中上調的MicroRNA有9箇，下調的MicroRNA有29箇；雌性假手術組與雄性假手術組對比髮現有差異錶達的MicroRNA有102箇，其中上調MicroRNA的有22箇，下調的MicroRNA有80箇。結果說明，不同性彆小鼠腎缺血再灌註前後腎組織中微小覈糖覈痠錶達存在差異，這些 MicroRNA 的差異錶達可能導緻不同性彆小鼠腎髒對缺血再灌註損傷的敏感性及耐受性存在差異。
배경：유연구표명신결혈재관주손상존재성별차이，단기구체궤제유대진일보연구。<br> 목적：관찰자웅성소서결혈전후신장조직중MicroRNA표체급기차이，이기진일보연구MicroRNA재신결혈재관주손상성별차이중적작용급궤제。<br> 방법：장웅성화자성소서분별신결혈45 min재관주손상24 h，동시설치웅성화자성가수술조작대조。채용MicroRNA기인심편기술검측자웅소서신결혈45 min재관주손상24 h급가수술후신조직MicroRNA표체적차이，양품간표체차이적역치위2배。<br> 결과여결론：재자성신결혈재관주여웅성신결혈재관주조대비발현유표체상조적MicroRNA유5개；자성신결혈재관주조여자성가수술조대비발현유차이표체적MicroRNA유29개，기중상조적유MicroRNA유25개，하조적MicroRNA유4개；웅성신결혈재관주조여웅성가수술조대비발현유차이표체적MicroRNA유38개，기중상조적MicroRNA유9개，하조적MicroRNA유29개；자성가수술조여웅성가수술조대비발현유차이표체적MicroRNA유102개，기중상조MicroRNA적유22개，하조적MicroRNA유80개。결과설명，불동성별소서신결혈재관주전후신조직중미소핵당핵산표체존재차이，저사 MicroRNA 적차이표체가능도치불동성별소서신장대결혈재관주손상적민감성급내수성존재차이。
BACKGROUND:Renal ischemia-reperfusion injury has been shown to exhibit gender difference, but its precise mechanisms deserve further investigations. OBJECTIVE:To investigate the differential expression of microRNAs in the kidney between female and male mice in order to study the effects and mechanisms of microRNA in pathogenesis of ischemia-reperfusion injury between different genders. METHODS:Male and female mice received kidney ischemia for 45 minutes and reperfusion injury for 24 hours. Simultaneously, male and female sham surgery groups served as controls. The microRNA gene chip technology was used to detect the differences of microRNA expression in the kidney of male and female mice at 45 minutes after ischemia and 24 hours of reperfusion as wel as after sham surgery. The threshold of difference in expression among samples was double. RESULTS AND CONCLUSION:Five microRNAs were up-regulated between female and male ischemia-reperfusion injury groups. Twenty-nine microRNAs differential y expressed in the female ischemia-reperfusion group and female sham surgery group, including 25 up-regulated microRNAs and 4 down-regulated microRNAs. Thirty-eight microRNAs differential y expressed in male ischemia-reperfusion injury group and male sham surgery group, including 9 up-regulated microRNAs and 29 down-regulated microRNAs. 102 microRNAs differential y expressed in the female sham surgery group and male sham surgery group, including 22 up-regulated microRNAs and 80 down-regulated microRNAs. Results suggested that there was differential expression in microRNAs in the kidney before and after renal ischemia-reperfusion in male and female mice. These differential y expressed microRNAs may be lead to different sensitivity and tolerance to the ischemia-reperfusion injury in the kidney of male and female mice.
