中国组织工程研究
中國組織工程研究
중국조직공정연구
Journal of Clinical Rehabilitative Tissue Engineering Research
2015年
5期
766-771
,共6页
张慧%黄立中%田莎%杨菊%戴新军
張慧%黃立中%田莎%楊菊%戴新軍
장혜%황립중%전사%양국%대신군
实验动物%消化系统损伤模型%六神丸%食管癌%抑瘤作用%血管内皮生长因子%微血管密度
實驗動物%消化繫統損傷模型%六神汍%食管癌%抑瘤作用%血管內皮生長因子%微血管密度
실험동물%소화계통손상모형%륙신환%식관암%억류작용%혈관내피생장인자%미혈관밀도
Esophageal Neoplasms%Vascular Endothelial Growth Factors%Drugs,Chinese Herbal
背景：六神丸是传统中药复方，具有清热解毒，散结消肿止痛之功效。现代药理证明六神丸具有抗炎、镇痛、强心、抗病毒、抗肿瘤等作用，临床上常用于各类感染性疾病、恶性肿瘤的治疗。
<br> 目的：探讨六神丸对食管癌移植瘤的抑瘤作用及对微血管密度、血管内皮生长因子表达的影响。
<br> 方法：通过复制人食管癌裸鼠移植瘤模型，将48只裸鼠随机分为六神丸高剂量组、六神丸中剂量组、六神丸低剂量组、顺铂组、模型组、空白组，按照给药方案用药。用药20 d后处死动物，称量瘤体质量，计算抑瘤率；免疫组化检测瘤组织微血管密度、血管内皮生长因子的表达。
<br> 结果与结论：六神丸高、中、低剂量组、顺铂组的移植瘤质量明显低于模型组，差异有显著性意义(P<0.05)。六神丸高、中、低剂量各组移植瘤内微血管密度、血管内皮生长因子的表达明显低于模型组，但没有顺铂组明显。结果表明六神丸对食管癌移植瘤生长有明显抑制作用，通过下调肿瘤组织血管内皮生长因子表达活性、降低微血管密度，可能是六神丸抑瘤作用机制之一。
揹景：六神汍是傳統中藥複方，具有清熱解毒，散結消腫止痛之功效。現代藥理證明六神汍具有抗炎、鎮痛、彊心、抗病毒、抗腫瘤等作用，臨床上常用于各類感染性疾病、噁性腫瘤的治療。
<br> 目的：探討六神汍對食管癌移植瘤的抑瘤作用及對微血管密度、血管內皮生長因子錶達的影響。
<br> 方法：通過複製人食管癌裸鼠移植瘤模型，將48隻裸鼠隨機分為六神汍高劑量組、六神汍中劑量組、六神汍低劑量組、順鉑組、模型組、空白組，按照給藥方案用藥。用藥20 d後處死動物，稱量瘤體質量，計算抑瘤率；免疫組化檢測瘤組織微血管密度、血管內皮生長因子的錶達。
<br> 結果與結論：六神汍高、中、低劑量組、順鉑組的移植瘤質量明顯低于模型組，差異有顯著性意義(P<0.05)。六神汍高、中、低劑量各組移植瘤內微血管密度、血管內皮生長因子的錶達明顯低于模型組，但沒有順鉑組明顯。結果錶明六神汍對食管癌移植瘤生長有明顯抑製作用，通過下調腫瘤組織血管內皮生長因子錶達活性、降低微血管密度，可能是六神汍抑瘤作用機製之一。
배경：륙신환시전통중약복방，구유청열해독，산결소종지통지공효。현대약리증명륙신환구유항염、진통、강심、항병독、항종류등작용，림상상상용우각류감염성질병、악성종류적치료。
<br> 목적：탐토륙신환대식관암이식류적억류작용급대미혈관밀도、혈관내피생장인자표체적영향。
<br> 방법：통과복제인식관암라서이식류모형，장48지라서수궤분위륙신환고제량조、륙신환중제량조、륙신환저제량조、순박조、모형조、공백조，안조급약방안용약。용약20 d후처사동물，칭량류체질량，계산억류솔；면역조화검측류조직미혈관밀도、혈관내피생장인자적표체。
<br> 결과여결론：륙신환고、중、저제량조、순박조적이식류질량명현저우모형조，차이유현저성의의(P<0.05)。륙신환고、중、저제량각조이식류내미혈관밀도、혈관내피생장인자적표체명현저우모형조，단몰유순박조명현。결과표명륙신환대식관암이식류생장유명현억제작용，통과하조종류조직혈관내피생장인자표체활성、강저미혈관밀도，가능시륙신환억류작용궤제지일。
BACKGROUND:Liushen Pil is a traditional Chinese herbal formula, has the effects of heat-clearing and detoxicating, eliminating stagnation, detumescence and al eviating pain. Modern pharmacology verifies that Liushen Pil has anti-inflammatory, analgesic, cardiac, anti-viral, anti-tumor effects, and has been extensively used in the treatment of various infectious diseases and malignant cancer. OBJECTIVE:To investigate the inhibitory effects of Liushen Pil on esophageal cancer xenografts, and effects on microvessel density and vascular endothelial growth factor expression. METHODS:After reproducing nude mouse models of human esophageal cancer, 48 nude mice were randomly divided into high-dose Liushen Pil group, moderate-dose Liushen Pil group, low-dose Liushen Pil group, cisplatin group, model group and blank group. According to medication regimen, drugs were given. The growth of transplanted tumor of nude mice was dynamical y observed in each group. The nude mice were sacrificed after 20 days of treatment. Neoplasm weight was taken and the tumor-suppressing rate was calculated. Immunohistochemistry was used to detect microvessel density and the expression of vascular endothelial growth factor. RESULTS AND CONCLUSION:The weight of transplanted tumor was significantly lower in the high-dose Liushen Pil group, moderate-dose Liushen Pil group, low-dose Liushen Pil group, and cisplatin group than in the model group (P<0.05). Microvessel density and the expression of vascular endothelial growth factor were obviously lower in the each Liushen Pil group than in the model group, but not as apparent as that in the cisplatin group. Results suggested that Liushen Pil can inhibit the growth of the esophageal cancer xenografts. Liushen Pil can down-regulate the expression of vascular endothelial growth factor and reduce microvessel density, which is one of the tumor-inhibiting mechanism of Liushen Pil .
