CAJ | 학술논문

背景：基质蛋白是构成血管壁的必不可少的重要成分，为血管壁的完整性和血管壁细胞发挥生理功能提供了必要的框架，并参与对细胞和平滑肌的调控。 　　目的：构建早期动脉瘤模型大鼠评价基质结构蛋白在形成过程中的表达差异性。 　　方法：将28只健康雄性SD大鼠随机分为2组：对照组(n=8)和模型组(n=20)。模型组大鼠以结扎左侧颈总动脉和右侧肾肾动脉致高血压方法建立脑动脉瘤模型；对照组大鼠仅暴露左侧颈动脉分叉和双侧颈动脉。模型组大鼠分别于造模后15，30 d处死，取大鼠右侧大脑前动脉和嗅动脉的分叉处部分组织进行免疫组化染色，分析纤维连接蛋白、α-平滑肌肌动蛋白和Ⅲ型胶原蛋白的表达。 　　结果与结论：与对照组相比，造模后30 d时模型组大鼠右侧大脑前动脉和嗅动脉的分叉处部分中纤维连接蛋白表达水平差异无显著性意义(P>0.05)，而Ⅲ型胶原和α-平滑肌肌动蛋白表达明显减少(P<0.05)。提示大鼠早期脑动脉瘤形成过程中的结构蛋白表达存在差异性并发生动态变化，血管壁基质结构蛋白降解是动脉瘤形成主要机制之一。
배경：기질단백시구성혈관벽적필불가소적중요성분，위혈관벽적완정성화혈관벽세포발휘생리공능제공료필요적광가，병삼여대세포화평활기적조공。 　　목적：구건조기동맥류모형대서평개기질결구단백재형성과정중적표체차이성。 　　방법：장28지건강웅성SD대서수궤분위2조：대조조(n=8)화모형조(n=20)。모형조대서이결찰좌측경총동맥화우측신신동맥치고혈압방법건립뇌동맥류모형；대조조대서부폭로좌측경동맥분차화쌍측경동맥。모형조대서분별우조모후15，30 d처사，취대서우측대뇌전동맥화후동맥적분차처부분조직진행면역조화염색，분석섬유련접단백、α-평활기기동단백화Ⅲ형효원단백적표체。 　　결과여결론：여대조조상비，조모후30 d시모형조대서우측대뇌전동맥화후동맥적분차처부분중섬유련접단백표체수평차이무현저성의의(P>0.05)，이Ⅲ형효원화α-평활기기동단백표체명현감소(P<0.05)。제시대서조기뇌동맥류형성과정중적결구단백표체존재차이성병발생동태변화，혈관벽기질결구단백강해시동맥류형성주요궤제지일。
BACKGROUND:Matrix protein is an essential component of the vascular wal , provides a necessary frame for the integrity of the vessel wal and physiological function of vascular wal cel s, and regulates cel s and smooth muscle. OBJECTIVE:To construct rat model of early aneurysm, and to evaluate differences in the expression of matrix structural proteins during cerebral aneurysm formation. METHODS:Twenty-eight healthy male Sprague-Dawley rats were randomized into control group (n=8) and model group (n=20). Aneurysm model was established by ligation of the left common carotid artery and right renal artery-induced hypertension in the model group. In the control group, only the left carotid artery bifurcation and bilateral carotid were exposed in rats. Rats in the model group were sacrificed at 15 and 30 days after model establishment. Right anterior cerebral artery in rats and olfactory artery bifurcation received immunohistochemical staining. The expressions of fibronectin,α-smooth muscle actin and col agen III were analyzed. RESULTS AND CONCLUSION:Compared with the control group, no significant difference in fibronectin expression was detected in right anterior cerebral artery and olfactory artery bifurcation in rats of the model group at 30 days after model establishment (P>0.05). However,α-smooth muscle actin and col agen III expressions were significantly reduced (P<0.05). These data confirmed that expression of structural proteins had differences and dynamic changes during early aneurysm formation in rats. Degradation of matrix structural protein in cerebral artery may be one of the key mechanism of aneurysm formation.