科技创新导报
科技創新導報
과기창신도보
SCIENCE AND TECHNOLOGY CONSULTING HERALD
2015年
4期
1-1,3
,共2页
金莲花%总黄酮%紫外分光光度法%正交试验法
金蓮花%總黃酮%紫外分光光度法%正交試驗法
금연화%총황동%자외분광광도법%정교시험법
Trollius Chinensis%Total Flavones%UV-spectrophotometry%Orthogonal Design
目的：研究金莲花提取工艺。方法采用正交试验法对金莲花中总黄酮进行提取，以出膏率和紫外分光光度法测定的总黄酮得率为指标，考察乙醇浓度（A）、回流时间（B、回流次数（C）、料液比（D）4个因素。检测波长为500 nm。结果优化的金莲花提取条件为A2B1C3D2。结论采用65%乙醇，料液比为1∶10，回流3次，每次2小时为最佳工艺，其中回流次数为显著性因素。黄酮最高含量为15.74%，最高出膏率为45.72%。
目的：研究金蓮花提取工藝。方法採用正交試驗法對金蓮花中總黃酮進行提取，以齣膏率和紫外分光光度法測定的總黃酮得率為指標，攷察乙醇濃度（A）、迴流時間（B、迴流次數（C）、料液比（D）4箇因素。檢測波長為500 nm。結果優化的金蓮花提取條件為A2B1C3D2。結論採用65%乙醇，料液比為1∶10，迴流3次，每次2小時為最佳工藝，其中迴流次數為顯著性因素。黃酮最高含量為15.74%，最高齣膏率為45.72%。
목적：연구금연화제취공예。방법채용정교시험법대금연화중총황동진행제취，이출고솔화자외분광광도법측정적총황동득솔위지표，고찰을순농도（A）、회류시간（B、회류차수（C）、료액비（D）4개인소。검측파장위500 nm。결과우화적금연화제취조건위A2B1C3D2。결론채용65%을순，료액비위1∶10，회류3차，매차2소시위최가공예，기중회류차수위현저성인소。황동최고함량위15.74%，최고출고솔위45.72%。
Objective to study on extraction process of Trollius Chinensis.Methods The study was carried out through Orthogonal Design,the congtent of Orientin determined by UV-spectrophotometry and the rate of obtaining paste were as guide.4 influencial factors,ethanol concentration(A),time of extraction(B),return the number(C)and material liquid than(D)were studied.The detection wavelength was 500 nm. Results The optimal conditions for the extraction were A2B1C3D2.Conclusion Extract three times by 65% ethanol,2 hours for every time,the material liquid than was 1:10,and the notable factor was return the number. The highest content of total flavones was 15.74%,and the highest extract rate was 45.72%.
