动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年
3期
120-123
,共4页
孔令芸%陈玲丽%李冲%孙相和%李鹏%王选年%银梅
孔令蕓%陳玲麗%李遲%孫相和%李鵬%王選年%銀梅
공령예%진령려%리충%손상화%리붕%왕선년%은매
猪瘟病毒%免疫细胞化学%反转录-聚合酶链反应%序列分析
豬瘟病毒%免疫細胞化學%反轉錄-聚閤酶鏈反應%序列分析
저온병독%면역세포화학%반전록-취합매련반응%서렬분석
Classical swine fever virus%cell immunochemistry%RT-PCR%sequence analysis
新乡市某猪场发生疑似猪瘟病例，为了鉴别其为疫苗毒还是野毒，采用细胞免疫化学方法和RT-PCR，并对其NS5B基因进行序列测定，序列比对并构建进化树，结果表明，猪只为猪瘟野毒感染，且分离的猪瘟病毒与石门（Shimen）株在基因序列上未发生大的变异。
新鄉市某豬場髮生疑似豬瘟病例，為瞭鑒彆其為疫苗毒還是野毒，採用細胞免疫化學方法和RT-PCR，併對其NS5B基因進行序列測定，序列比對併構建進化樹，結果錶明，豬隻為豬瘟野毒感染，且分離的豬瘟病毒與石門（Shimen）株在基因序列上未髮生大的變異。
신향시모저장발생의사저온병례，위료감별기위역묘독환시야독，채용세포면역화학방법화RT-PCR，병대기NS5B기인진행서렬측정，서렬비대병구건진화수，결과표명，저지위저온야독감염，차분리적저온병독여석문（Shimen）주재기인서렬상미발생대적변이。
The suspicious cases of classical swine fever occurred in a pig farm of Xinxiang.The virus was i-dentified by cell immunochemistry method and RT-PCR.The results of the sequence alignment and phylo-genetic tree of NS5B gene showed that the pigs were infected with classical swine fever virus field strain, and the gene sequence variation of this isolate was not obvious.
