CAJ | 학술논문

探讨体外能量物质对 SD大鼠快肌（胫前肌）与慢肌（股中间肌）卫星细胞增殖功能和 H2O2化学损伤修复研究。采用快、慢肌卫星细胞传代培养及分离纯化，四唑盐（MTT）比色法、DAPI荧光染色与免疫荧光抗体检测。结果表明：①与正常组比较，低剂量或中等剂量能量物质对快肌与慢肌卫星细胞有增殖机能，各剂量作用时间24 h＞48 h＞72 h，其中以ATP溶液1．25 mg／mL，ADP溶液1．25 mg／mL，AMP溶液2．5 mg／mL剂量促进快、慢肌卫星细胞增殖效果最显著；② MTT比色法检测ATP、ADP及AMP修复损伤快、慢肌卫星细胞明显低于正常组卫星细胞而高于纯 H2 O2损伤卫星细胞。且修复效果表现为：ATP溶液以1．25 mg／mL，ADP溶液为2．5 mg／mL，AMP溶液为 2．5 mg／mL修复最佳；③模型组 Bax抗体细胞数102．5±5．7，Bcl-2抗体细胞29．6±3．7；ATP、ADP 及 AMP 溶液保护组 Bax 抗体细胞数67．3±4．9～72．8±5．4范围，而Bcl-2抗体细胞在69．8±5．1～78．5±4．9范围之内。慢肌股中间肌卫星细胞各组检测结果与其类似。
탐토체외능량물질대 SD대서쾌기（경전기）여만기（고중간기）위성세포증식공능화 H2O2화학손상수복연구。채용쾌、만기위성세포전대배양급분리순화，사서염（MTT）비색법、DAPI형광염색여면역형광항체검측。결과표명：①여정상조비교，저제량혹중등제량능량물질대쾌기여만기위성세포유증식궤능，각제량작용시간24 h＞48 h＞72 h，기중이ATP용액1．25 mg／mL，ADP용액1．25 mg／mL，AMP용액2．5 mg／mL제량촉진쾌、만기위성세포증식효과최현저；② MTT비색법검측ATP、ADP급AMP수복손상쾌、만기위성세포명현저우정상조위성세포이고우순 H2 O2손상위성세포。차수복효과표현위：ATP용액이1．25 mg／mL，ADP용액위2．5 mg／mL，AMP용액위 2．5 mg／mL수복최가；③모형조 Bax항체세포수102．5±5．7，Bcl-2항체세포29．6±3．7；ATP、ADP 급 AMP 용액보호조 Bax 항체세포수67．3±4．9～72．8±5．4범위，이Bcl-2항체세포재69．8±5．1～78．5±4．9범위지내。만기고중간기위성세포각조검측결과여기유사。
The repair study of H2 O2 chemical injury and value-added function were investigated with energy substances for fast tibialis anterior muscle and slow vastus intermedius muscle satellite cells invitro at SD rat.The satellite cells of fast and slow muscle were cultured by primary subculture and purification.The tetrazolium salt (MTT)assay,DAPI staining and immunofluorescence antibody were used for detection. The results indiciated:① To compared with the normal group,low-dose or medium-dose ATP,ADP,and AMP were significant for satellite cell proliferation of fast and slow muscle in a variety of dosage time was 24 h>48 h>72 h.ATP solution 1.25 mg/mL,ADP solution 1.25 mg/mL,AMP solution 2.5 mg/mL dose facilitated fast and slow muscle satellite cell proliferation in the most significant effect.② ATP, ADP,and AMP were found to repair fast muscle satellite cells damaged with tetrazolium detected by MTT assay,its activity was significantly lower than the normal group of satellite cells and was higher than pure H2 O2 injury satellite cells.And the restore effect of performance:ATP solution 1.25 mg/mL,ADP solu-tion 2.5 mg/mL,AMP solution of 2.5 mg/mL were best restoration.③ Bax antibody cells in model group were 102.5±5.7,Bcl-2 antibody cell number were 29.6±3.7;Bax antibody cells in ATP,ADP and AMP solution protective group were 67.3±4.9-72.8±5.4 range,and Bcl-2 antibody cells 69.8±5.1-78.5±4.9 range.The results of slow muscle satellite cell groups tested were similar.