CAJ | 학술논문

2013年下半年，福建某免疫接种过猪伪狂犬病疫苗（Bartha）的规模化猪场大批妊娠母猪发生流产、新生仔猪发生共济失调的神经症状，疑似为猪伪狂犬病病毒感染发病症状，为确定发病原因，从该猪场疑似伪狂犬病病毒感染的仔猪脑、肝脏和肺脏中分离到一株未知病毒，PCR检测及测序比对鉴定为猪伪狂犬病病毒，并将分离的病毒命名为 NP 株。用 Reed-Muench 法测定分离株病毒的组织细胞半数感染量（TCID50）为10－9．13／0．1 mL，动物攻毒试验出现猪伪狂犬病病毒感染的典型症状。试验结果表明，成功分离到一株猪伪狂犬病病毒毒株，为研究福建省猪群伪狂犬病病毒分子流行病学奠定基础。
2013년하반년，복건모면역접충과저위광견병역묘（Bartha）적규모화저장대비임신모저발생유산、신생자저발생공제실조적신경증상，의사위저위광견병병독감염발병증상，위학정발병원인，종해저장의사위광견병병독감염적자저뇌、간장화폐장중분리도일주미지병독，PCR검측급측서비대감정위저위광견병병독，병장분리적병독명명위 NP 주。용 Reed-Muench 법측정분리주병독적조직세포반수감염량（TCID50）위10－9．13／0．1 mL，동물공독시험출현저위광견병병독감염적전형증상。시험결과표명，성공분리도일주저위광견병병독독주，위연구복건성저군위광견병병독분자류행병학전정기출。
In mid 2013,one strain of pseudorabies virus (PRV)was isolated from organs of dead piglets with symptoms of PRV-like infection,which were collected from a large-scale farms in Fuj ian Province,it was named PRV NP strain.The symptoms of PRV infection were characterized by nervous system disor-ders and deaths in newborn piglets as well as reproductive failure in sows.In addition,the PCR and se-quencing results were consistent with expected results;PRV was able to propagate in MDCK cells and cause a typical cytopathogenic effect.The tissue culture infective dose (TCID50)was 10-9.13/0.1 mL con-firmed by the Reed-Muench methods.The typical pseudorabies clinical symptoms were presented in rabbits and mice inoculated with the isolated virus.These results demonstrated that a new wild pseudorabies virus has been isolated,which lay a foundation for the further research on the molecular epidemiology of pseud-orabies virus.