CAJ | 학술논문

为研究丹参水提物（SME）对子宫内膜上皮细胞炎症模型中基质金属蛋白酶-2（MMP-2）表达的影响，体外培养山羊子宫内膜上皮细胞（EEC），利用内毒素（脂多糖，LPS）诱导细胞增殖和产生TNF-α，建立EEC炎症模型。然后给予高、中、低不同剂量 SME干预。应用 MTT比色法检测细胞活力，ELISA试剂盒检测TNF-α含量，实时荧光定量 PCR检测细胞 MMP-2 mRNA表达变化。结果表明，5μg／mL LPS作用EEC 12 h时促增殖作用显著（P＜0．01）；SME可显著促进EEC炎症模型中MMP-2的表达，以中、高浓度组的作用较为明显。说明SME对LPS引起的子宫内膜炎症反应有一定的保护作用。
위연구단삼수제물（SME）대자궁내막상피세포염증모형중기질금속단백매-2（MMP-2）표체적영향，체외배양산양자궁내막상피세포（EEC），이용내독소（지다당，LPS）유도세포증식화산생TNF-α，건립EEC염증모형。연후급여고、중、저불동제량 SME간예。응용 MTT비색법검측세포활력，ELISA시제합검측TNF-α함량，실시형광정량 PCR검측세포 MMP-2 mRNA표체변화。결과표명，5μg／mL LPS작용EEC 12 h시촉증식작용현저（P＜0．01）；SME가현저촉진EEC염증모형중MMP-2적표체，이중、고농도조적작용교위명현。설명SME대LPS인기적자궁내막염증반응유일정적보호작용。
To observe the effects of Salviamiltiorrhiza extract (SME)on the expression of matrix metal-loproteinase-2 (MMP-2)in goat endometrial epithelial cells(EEC)with inflammation induced by lipopo-lysaccharide (LPS),the high,medium and low dosages of SME were used to intervene to the MMP-2 mR-NA expression.The viability of cells,concentration of TNF-αand MMP-2 mRNA expression were detected by MTT colorimetry,ELISA kits and real-time PCR respectively.It turned out that the proliferation of cells had been improved significantly when LPS(5μg/mL)stimulated EEC for 1 2 h.And the expression of MMP-2 was up-regulated by SME,especially in medium dosage and high dosage.SME could decrease en-dometrial inflammatory response caused by LPS.