CAJ | 학술논문

为了探讨黄芪水煎液能否诱导大鼠肠黏膜微血管内皮细胞（RIMVECs）表达IFN-γ，采用MTT和双夹心ELISA分别检测了黄芪水煎液对体外培养的 RIMMVECs增殖以及表达 IFN-γ的影响。结果显示，黄芪水煎液在25 mg／L～3200 mg／L时促进 RIMVECs增殖。为更好的研究黄芪水煎液诱导效果，将不同浓度的黄芪水煎液（0．1、1、6、30 mg／mL）诱导 RIMVECs 6、12、24 h检测 IFN-γ含量，结果显示，在各检测时间点0．1、1 mg／mL组和空白组差异显著（P＜0．05），6 mg／mL组差异极显著（P＜0．01），30 mg／mL组差异不显著（P＞0．05）。说明黄芪水煎液的抗病毒作用可能与诱导微血管内皮细胞表达 IFN-γ有直接的关系，为黄芪“益气解毒”功效的现代阐释提供了试验依据。
위료탐토황기수전액능부유도대서장점막미혈관내피세포（RIMVECs）표체IFN-γ，채용MTT화쌍협심ELISA분별검측료황기수전액대체외배양적 RIMMVECs증식이급표체 IFN-γ적영향。결과현시，황기수전액재25 mg／L～3200 mg／L시촉진 RIMVECs증식。위경호적연구황기수전액유도효과，장불동농도적황기수전액（0．1、1、6、30 mg／mL）유도 RIMVECs 6、12、24 h검측 IFN-γ함량，결과현시，재각검측시간점0．1、1 mg／mL조화공백조차이현저（P＜0．05），6 mg／mL조차이겁현저（P＜0．01），30 mg／mL조차이불현저（P＞0．05）。설명황기수전액적항병독작용가능여유도미혈관내피세포표체 IFN-γ유직접적관계，위황기“익기해독”공효적현대천석제공료시험의거。
To study the effect of Astragalus aqua on interferon-γ(IFN-γ)expression in intestinal microvascular endo-thelial cells (IMMVECs),rat intestinal mucosa microvascular endothelial cells (RIMMVECs)were co-cultured with Astragalus aqua,and the cell proliferation and IFN-γexpression level were examined by MTT and sandwich ELISA, respectively.The results showed that the proliferation of RIMMVECs was increased by Astragalus aqua at different concentrations ranged between 25 mg/L and 3 200 mg/L.The effect of IFN-γexpression induced by Astragalus aqua was further explored;RIMMVECs were co-cultured with Astragalus aqua at concentrations of 0.1,1,6,30 mg/mL for 6,12,24 hours,respectively.The expression of IFN-γwas significantly increased by Astragalus aqua at concentrations of 0.1,1 mg/mL at each time point compared to that of blank group.And the expression of IFN-γwas even more sig-nificantly increased by Astragalus aqua at concentration of 6 mg/mL;whereas,no significant increase by Astragalus aqua was observed at concentration of 30 mg/mL compared to that of blank control.Our findings indicated that the anti-virus effect of Astragalus aqua might be due to its induction effect on microvascular endothelial cells to produce IFN-γ,which offered the molecular biology evidence to interpret its'immune-resistance enhancing and disintoxication'effect as an ancient Chinese herb.