CAJ | 학술논문

研究分析了牦牛皮蝇幼虫蛋白表达谱，为牛皮蝇的基础研究和蛆病防控提供数据。试验提取了从牦牛皮下采集的中华皮蝇（H．sinense）幼虫总蛋白，SDS-PAGE分析幼虫蛋白表达谱，用分子筛和离子交换层析对一种高表达蛋白进行纯化，并对表达谱中差异极大的两个样品进行双向电泳和质谱分析。结果显示，幼虫个体间蛋白表达谱存在不同程度差异，约23％的个体中含有一种分子质量约为78 ku的高表达蛋白，纯化并获得了该蛋白质；双向电泳分别获得了312±24和284±13个蛋白点，但对其中的4个蛋白点进行质谱分析，未得到分数高的匹配结果。研究获得了牛皮蝇幼虫蛋白表达谱和纯化的蛋白质，为进一步研究牛皮蝇幼虫的基因表达特征、蛋白质功能等奠定了基础。
연구분석료모우피승유충단백표체보，위우피승적기출연구화저병방공제공수거。시험제취료종모우피하채집적중화피승（H．sinense）유충총단백，SDS-PAGE분석유충단백표체보，용분자사화리자교환층석대일충고표체단백진행순화，병대표체보중차이겁대적량개양품진행쌍향전영화질보분석。결과현시，유충개체간단백표체보존재불동정도차이，약23％적개체중함유일충분자질량약위78 ku적고표체단백，순화병획득료해단백질；쌍향전영분별획득료312±24화284±13개단백점，단대기중적4개단백점진행질보분석，미득도분수고적필배결과。연구획득료우피승유충단백표체보화순화적단백질，위진일보연구우피승유충적기인표체특정、단백질공능등전정료기출。
The aim of this study was to reveal the protein expression profiling of the Hypoderma larva in-fecting yaks,in order to provide data for the basic research of Hypoderma and the prevention of hypoder-mosis.Total protein of the Hypodermasinense larva collected from subcutaneous tissues of yak was extrac-ted and analyzed by SDS-PAGE.A protein with high was purified using gel exlusion and ion-exchange chromatography and two samples of Hypoderma larvae whose total protein profiling displayed great differ-ence were separated and identified by two-dimensional electrophoresis and mass spectrometry.The results showed that the protein expression profile of larvae varied among individuals,and approximately 23% of the individuals contained a protein with molecular weight of about 78 ku,which was purified by above methods.A total of 312±24 and 284±13 protein spots were obtained respectively by two-dimensional e-lectrophoresis and four of these proteins were analyzed by mass spectrometry,however,none of the pro-teins were identified with high score matches.This study obtained the protein expression profiling of Hy-poderma larvae and purified a protein,laying a foundation for further studies on the gene expression and protein functions of Hypoderma larvae.