动物医学进展
動物醫學進展
동물의학진전
PROGRESS IN VETERINARY MEDICINE
2015年
3期
13-16
,共4页
王培坤%毕玉彧%秦丽莉%邹广珍%彭昊%韦平
王培坤%畢玉彧%秦麗莉%鄒廣珍%彭昊%韋平
왕배곤%필옥욱%진려리%추엄진%팽호%위평
共感染%A亚群禽白血病病毒%J亚群禽白血病病毒%gp85%聚合酶链反应
共感染%A亞群禽白血病病毒%J亞群禽白血病病毒%gp85%聚閤酶鏈反應
공감염%A아군금백혈병병독%J아군금백혈병병독%gp85%취합매련반응
co-infection%ALV-J%ALV-A%gp85%PCR
为了解禽白血病病毒在广西鸡群中流行情况,采用 DF-1细胞接种、细胞培养上清 p27抗原检测、PCR扩增,对临床血管瘤型禽白血病的三黄鸡病料进行了病毒分离,并对分离病毒 gp85基因进行测序和序列比较。结果表明,从1只病鸡同时分离到了一株A亚群禽白血病病毒(ALV-A)与一株J亚群禽白血病病毒(ALV-J),分别命名为 HG01-A 株和 HG01-J 株。ALV-A gp85与7株 A 亚群氨基酸同源性为85.8%~87.5%,与 A 亚群美国株 MQNCSU 同源性最高为 87.5%,与 A 亚群原型株 RSA 同源性为86.9%。而ALV-J gp85与7株毒株核酸序列同源性为84.0%~93.8%,与广东株XX2-08以及四川株SC-SM01同源性最高为93.8%,与英国原型株 HPRS103同源性为90.2%。进化分析进一步表明,HG01-A与各参考株亲缘关系较远,H G01-J与SCSM01 亲缘关系最近。本研究首次从同一只广西三黄鸡中同时分离到ALV-A及ALV-J,进一步完善了我国地方品种鸡群中禽白血病的流行病学信息。
為瞭解禽白血病病毒在廣西鷄群中流行情況,採用 DF-1細胞接種、細胞培養上清 p27抗原檢測、PCR擴增,對臨床血管瘤型禽白血病的三黃鷄病料進行瞭病毒分離,併對分離病毒 gp85基因進行測序和序列比較。結果錶明,從1隻病鷄同時分離到瞭一株A亞群禽白血病病毒(ALV-A)與一株J亞群禽白血病病毒(ALV-J),分彆命名為 HG01-A 株和 HG01-J 株。ALV-A gp85與7株 A 亞群氨基痠同源性為85.8%~87.5%,與 A 亞群美國株 MQNCSU 同源性最高為 87.5%,與 A 亞群原型株 RSA 同源性為86.9%。而ALV-J gp85與7株毒株覈痠序列同源性為84.0%~93.8%,與廣東株XX2-08以及四川株SC-SM01同源性最高為93.8%,與英國原型株 HPRS103同源性為90.2%。進化分析進一步錶明,HG01-A與各參攷株親緣關繫較遠,H G01-J與SCSM01 親緣關繫最近。本研究首次從同一隻廣西三黃鷄中同時分離到ALV-A及ALV-J,進一步完善瞭我國地方品種鷄群中禽白血病的流行病學信息。
위료해금백혈병병독재엄서계군중류행정황,채용 DF-1세포접충、세포배양상청 p27항원검측、PCR확증,대림상혈관류형금백혈병적삼황계병료진행료병독분리,병대분리병독 gp85기인진행측서화서렬비교。결과표명,종1지병계동시분리도료일주A아군금백혈병병독(ALV-A)여일주J아군금백혈병병독(ALV-J),분별명명위 HG01-A 주화 HG01-J 주。ALV-A gp85여7주 A 아군안기산동원성위85.8%~87.5%,여 A 아군미국주 MQNCSU 동원성최고위 87.5%,여 A 아군원형주 RSA 동원성위86.9%。이ALV-J gp85여7주독주핵산서렬동원성위84.0%~93.8%,여엄동주XX2-08이급사천주SC-SM01동원성최고위93.8%,여영국원형주 HPRS103동원성위90.2%。진화분석진일보표명,HG01-A여각삼고주친연관계교원,H G01-J여SCSM01 친연관계최근。본연구수차종동일지엄서삼황계중동시분리도ALV-A급ALV-J,진일보완선료아국지방품충계군중금백혈병적류행병학신식。
In order to understand the avian leukosis virus epidemic situation in Guangxi chickens,two strains of avian leukosis virus subgroup A and J were isolated in Three-yellow chickens in the cultured DF-1 cells,which were identified by ALV p2 7 detection and PCR amplification and sequence analysis of avian leukosis virus gp85 gene,and then were named as HG01-A and HG01-J,respectively.The results of varia-tion analysis of gp85 gene indicated that the homology between HG01-A and 7 reference strains was 85.8%-87.5%,and shared the highest homology with MQNCSU at 87.5%,shared the homology with RSA at 86.9%.The results of variation analysis of gp85 gene indicated that the homology between HG01-J and 7 reference strains was 84.0%-93.8%,and shared the highest homology withXX2-08 and SCSM01 at 92.8%,shared the homology withHPRS1-3 at 90.2%.The present results indicated that it the first report that there was the co-infection of ALV-A and ALV-J in the Guangxi Three-yellow chickens,and provided new epidemiological information of avian leucosis virus in local chicken.