CAJ | 학술논문

目的：探讨天芪益智颗粒对阿尔茨海默病（alzheimer’s disease, AD）大鼠学习记忆的保护作用及对脑组织氧化应激的影响。方法90只雄性SD大鼠按随机数字表法分为假手术组，模型组，石杉碱甲组以及天芪益智低、中、高剂量组，每组15只。右侧侧脑室注射β-淀粉样多肽1-42(Aβ1-42)制备AD模型。造模1周后开始给药，天芪益智颗粒高、中、低剂量组分别灌胃天芪益智颗粒溶液生药含量分别为0.8、1.6和3.2 g/kg；石杉碱甲组灌胃石杉碱甲溶液0.02 mg/kg；假手术组和模型组灌胃等体积生理盐水。连续灌胃给药30 d。采用避暗实验检测大鼠学习记忆能力。检测脑组织超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GSH-Px）活性和丙二醛（MDA）水平。结果与模型组比较，天芪益智颗粒高、中剂量组进入暗室的潜伏期显著延长[(239.05±48.42)s、(214.35±74.52)s比(97.39±30.69）s；P均＜0.01]，错误次数显著减少[分别为(1.93±3.25)次、(2.27±3.09)次比(6.62±3.45)次；P 均＜0.05]，脑组织SOD[(177.27±63.10)U/mg、(164.53±72.58)U/mg比(72.56±21.04)U/mg；P均＜0.01]和 GSH-Px [(2899.36±362.27)U/g、(2407.68±472.14)U/g比(1397.64±442.17)U/g，P均＜0.01]活性显著增高， MDA 水平[(24.75±9.94)nmol/mg、(27.74±5.82)nmol/mg 比(37.56±17.23)nmol/mg，P 均＜0.01]显著降低。结论天芪益智颗粒可减轻AD大鼠脑组织氧化应激，改善学习记忆。
목적：탐토천기익지과립대아이자해묵병（alzheimer’s disease, AD）대서학습기억적보호작용급대뇌조직양화응격적영향。방법90지웅성SD대서안수궤수자표법분위가수술조，모형조，석삼감갑조이급천기익지저、중、고제량조，매조15지。우측측뇌실주사β-정분양다태1-42(Aβ1-42)제비AD모형。조모1주후개시급약，천기익지과립고、중、저제량조분별관위천기익지과립용액생약함량분별위0.8、1.6화3.2 g/kg；석삼감갑조관위석삼감갑용액0.02 mg/kg；가수술조화모형조관위등체적생리염수。련속관위급약30 d。채용피암실험검측대서학습기억능력。검측뇌조직초양화물기화매（SOD）、곡광감태과양화물매（GSH-Px）활성화병이철（MDA）수평。결과여모형조비교，천기익지과립고、중제량조진입암실적잠복기현저연장[(239.05±48.42)s、(214.35±74.52)s비(97.39±30.69）s；P균＜0.01]，착오차수현저감소[분별위(1.93±3.25)차、(2.27±3.09)차비(6.62±3.45)차；P 균＜0.05]，뇌조직SOD[(177.27±63.10)U/mg、(164.53±72.58)U/mg비(72.56±21.04)U/mg；P균＜0.01]화 GSH-Px [(2899.36±362.27)U/g、(2407.68±472.14)U/g비(1397.64±442.17)U/g，P균＜0.01]활성현저증고， MDA 수평[(24.75±9.94)nmol/mg、(27.74±5.82)nmol/mg 비(37.56±17.23)nmol/mg，P 균＜0.01]현저강저。결론천기익지과립가감경AD대서뇌조직양화응격，개선학습기억。
Objective To investigate the effects ofTianqi-Yizhi granules on oxidative stress in the brain tissue, and learning and memory in Alzheimer's disease model rats.Methods A total of 90 male SD rats were randomly divided into 6 groups by random number table method: sham operation group, model group, huperzine A group and groups of low-, medium- and high-doseTianqi-Yizhi granules, with 15 rats in each group. The AD rat model was prepared by the left lateral ventricle injection of amyloid-β1-42. One week after modeling, the rats in the groups of low-, medium- and high-doseTianqi-Yizhi granules received intragastric administration of 0.8, 1.6 and 3.2 g/kgTianqi-Yizhi granules, respectively; the rats in the huperzine A group received intragastric administration of 0.02 mg/kg huperzine A solution; and the rats in the sham operation and model groups received intragastric administration of equivalent volume of normal saline for 30 days. Learning and memory were evaluated using the dark avoidance test. The activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px), and malonaldehyde (MDA) level in the brain tissue were determined. ResultsIn comparison with the model group, the latencies to step into the dark chamber in the high- and medium-dose Tianqi-Yizhi granules groups were significantly longer (239.05 ± 48.42 s, 214.35 ± 74.52 svs. 97.39 ± 30.69 s; allP<0.01), the numbers of errors significantly decreased (1.93 ± 3.25, 2.27 ± 3.09vs. 6.62 ± 3.45; allP<0.05), the activities of SOD (177.27 ± 63.10 U/mg, 164.53 ± 72.58 U/mgvs.72.56±21.04 U/mg; all P<0.01) and GSH-Px (2 899.36 ± 362.27 U/g, 2 407.68 ± 472.14 U/gvs. 1 397.64 ± 442.17 U/g; allP<0.01), and MDA level (24.75 ± 9.94 nmol/mg, 27.74 ± 5.82 nmol/mgvs. 37.56 ± 17.23 nmol/mg; allP<0.01) in the brain tissue significantly increased. ConclusionTianqi-Yizhi granules could attenuate oxidative stress in the brain tissue, and improve learning and memory in AD rats.