国际中医中药杂志
國際中醫中藥雜誌
국제중의중약잡지
INTERNATIONAL JOURNAL OF TRIDITIONAL CHINESE MEDICINE
2015年
3期
235-238
,共4页
脑缺血%再灌注损伤%电针%促肾上腺皮质素释放激素%糖皮质激素%基因表达%大鼠
腦缺血%再灌註損傷%電針%促腎上腺皮質素釋放激素%糖皮質激素%基因錶達%大鼠
뇌결혈%재관주손상%전침%촉신상선피질소석방격소%당피질격소%기인표체%대서
Brain ischemia%Reperfusion injury%Electroacupuncture%Corticotropin-releasing hormone%Glucocorticoids%Gene expression%Rats
目的：探讨电针经穴对脑缺血再灌注损伤大鼠海马促肾上腺皮质激素释放因子（corticotropin-releasing factor, CRF）和皮质醇（corticosteroid, CORT）mRNA表达的影响。方法150只SD大鼠按随机数字表法分为经穴电针组、非经穴电针组、模型组、假手术组和正常组各30只。采用改良线栓法制备脑缺血再灌注模型。经穴电针组于脑缺血再灌注后6 h针刺双侧“曲池”“足三里”“百会”“风府”，1次/d，共7 d。脑缺血再灌注后1、3、7 d时评价神经功能缺损后，处死大鼠，取海马组织，利用实时荧光定量PCR检测海马CRF和CORT mRNA表达。结果脑缺血再灌注后1、3、7 d，模型组海马CRF和CORT mRNA[CRF mRNA表达分别为（1.122±0.249）、（1.190±0.666）、（0.454±0.612）， CORT mRNA表达分别为（0.917±0.113）、（1.024±0.290）、（0.709±0.055)]与假手术组[CRF mRNA分别为（0.021±0.049）、（0.021±0.027）、（0.035±0.005），CORT mRNA分别为（0.016±0.013）、（0.016±0.006）、（0.043±0.006)]比较差异有统计学意义(P均＜0.01)。经穴电针组CRF[（0.424±0.104）、（0.339±0.476）、（0.095±0.021）]和 CORT[0.377±0.073）、（0.138±0.025）、（0.158±0.010)]mRNA 表达较模型组显著下降(P均＜0.01)。脑缺血再灌注后1、3、7 d时，经穴电针组神经功能缺损评分为（1.83±0.75）分、（1.50±0.55）分和（1.17±0.41），显著低于模型组的（2.50±0.84）分、（2.33±0.52）和（1.67±0.52）分。结论电针经穴可降低脑缺血再灌注损伤大鼠海马CRF和CORT mRNA表达水平，改善神经功能缺损状态。
目的：探討電針經穴對腦缺血再灌註損傷大鼠海馬促腎上腺皮質激素釋放因子（corticotropin-releasing factor, CRF）和皮質醇（corticosteroid, CORT）mRNA錶達的影響。方法150隻SD大鼠按隨機數字錶法分為經穴電針組、非經穴電針組、模型組、假手術組和正常組各30隻。採用改良線栓法製備腦缺血再灌註模型。經穴電針組于腦缺血再灌註後6 h針刺雙側“麯池”“足三裏”“百會”“風府”，1次/d，共7 d。腦缺血再灌註後1、3、7 d時評價神經功能缺損後，處死大鼠，取海馬組織，利用實時熒光定量PCR檢測海馬CRF和CORT mRNA錶達。結果腦缺血再灌註後1、3、7 d，模型組海馬CRF和CORT mRNA[CRF mRNA錶達分彆為（1.122±0.249）、（1.190±0.666）、（0.454±0.612）， CORT mRNA錶達分彆為（0.917±0.113）、（1.024±0.290）、（0.709±0.055)]與假手術組[CRF mRNA分彆為（0.021±0.049）、（0.021±0.027）、（0.035±0.005），CORT mRNA分彆為（0.016±0.013）、（0.016±0.006）、（0.043±0.006)]比較差異有統計學意義(P均＜0.01)。經穴電針組CRF[（0.424±0.104）、（0.339±0.476）、（0.095±0.021）]和 CORT[0.377±0.073）、（0.138±0.025）、（0.158±0.010)]mRNA 錶達較模型組顯著下降(P均＜0.01)。腦缺血再灌註後1、3、7 d時，經穴電針組神經功能缺損評分為（1.83±0.75）分、（1.50±0.55）分和（1.17±0.41），顯著低于模型組的（2.50±0.84）分、（2.33±0.52）和（1.67±0.52）分。結論電針經穴可降低腦缺血再灌註損傷大鼠海馬CRF和CORT mRNA錶達水平，改善神經功能缺損狀態。
목적：탐토전침경혈대뇌결혈재관주손상대서해마촉신상선피질격소석방인자（corticotropin-releasing factor, CRF）화피질순（corticosteroid, CORT）mRNA표체적영향。방법150지SD대서안수궤수자표법분위경혈전침조、비경혈전침조、모형조、가수술조화정상조각30지。채용개량선전법제비뇌결혈재관주모형。경혈전침조우뇌결혈재관주후6 h침자쌍측“곡지”“족삼리”“백회”“풍부”，1차/d，공7 d。뇌결혈재관주후1、3、7 d시평개신경공능결손후，처사대서，취해마조직，이용실시형광정량PCR검측해마CRF화CORT mRNA표체。결과뇌결혈재관주후1、3、7 d，모형조해마CRF화CORT mRNA[CRF mRNA표체분별위（1.122±0.249）、（1.190±0.666）、（0.454±0.612）， CORT mRNA표체분별위（0.917±0.113）、（1.024±0.290）、（0.709±0.055)]여가수술조[CRF mRNA분별위（0.021±0.049）、（0.021±0.027）、（0.035±0.005），CORT mRNA분별위（0.016±0.013）、（0.016±0.006）、（0.043±0.006)]비교차이유통계학의의(P균＜0.01)。경혈전침조CRF[（0.424±0.104）、（0.339±0.476）、（0.095±0.021）]화 CORT[0.377±0.073）、（0.138±0.025）、（0.158±0.010)]mRNA 표체교모형조현저하강(P균＜0.01)。뇌결혈재관주후1、3、7 d시，경혈전침조신경공능결손평분위（1.83±0.75）분、（1.50±0.55）분화（1.17±0.41），현저저우모형조적（2.50±0.84）분、（2.33±0.52）화（1.67±0.52）분。결론전침경혈가강저뇌결혈재관주손상대서해마CRF화CORT mRNA표체수평，개선신경공능결손상태。
Objective To investigate the effects of electroacupuncture at meridian point on the mRNA expressions of corticotropin-releasing factor (CRF) and corticosteroid (CORT) in the hippocampus after cerebral ischemia reperfusion in rats.MethodsA total of 150 SD rats were randomly divided into 5 groups by random number table method: normal control group, sham operation group, model group, meridian point electroacupuncture group and non-meridian point electroacupuncture group, with 30 rats in each group. A model of focal cerebral ischemic reperfusion was induced using the modified intraluminal thread method. In the meridian point electroacupuncture group, at 6 h after cerebral ischemia reperfusion, acupuncture “LI11” on both sides, bilateral “ST36”, “GV20”, ”GV16” daily for 7 d. The neurological deficits were evaluated 1 d, 3 d, and 7 d after cerebral ischemia reperfusion, and then all rats were sacrificed and the hippocampi were harvested. The mRNA expressions of CRF and CORT in the hippocampus were determined by quantitative real-time PCR.ResultsThe mRNA expressions of CRF (1.122 ± 0.249, 1.190 ± 0.666, 0.454 ± 0.612 in the model group; 0.021 ± 0.049, 0.021 ± 0.027, 0.035 ± 0.005 in the sham operation group) and CORT (0.917 ± 0.113, 1.024 ± 0.290, 0.709 ± 0.055 in the model group; 0.016 ± 0.013, 0.016 ± 0.006, 0.043 ± 0.006 in the sham operation group) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly increased in the model group compared with the sham operation group (all P<0.01). The mRNA expressions of CRF (0.424 ± 0.104, 0.339 ± 0.476, 0.095 ± 0.021) and CORT (0.377 ± 0.073, 0.138 ± 0.025, 0.158 ± 0.010) in the hippocampus 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (allP<0.01). The neurological deficits scale 1 d, 3 d, 7 d after cerebral ischemic reperfusion were significantly decreased in the meridian point electroacupuncture group compared with the mode1 group (1.83 ± 0.75, 1.50 ± 0.55 and 1.17±0.41 in the meridian point electroacupuncture group; 2.50 ± 0.84, 2.33 ± 0.52 and 1.67 ± 0.52 in the model graoup; allP<0.01). Conclusion Electricacupuncture at meridian point can reduce the mRNA expressions of CRF and CORT in the hippocampus, and improve neurological deficits after cerebral ischemia reperfusion in rats.
